柱前衍生化-高效液相色谱法测定啤酒中的多胺  

Determination of polyamines in beer using high-performance liquid chromatography with derivatization reagent

作　　者：王利娜[1] 金东日[1] 

机构地区：[1]延边大学理学院化学系,吉林延吉133002

出　　处：《延边大学学报（自然科学版）》2014年第3期249-252,共4页Journal of Yanbian University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(21365022)

摘　　要：建立了用柱前衍生化高效液相色谱法分析啤酒中多胺的方法.在碱性介质中,多胺与1-(5-氟-2,4-二硝基苯基)-4-甲基哌嗪(PPZ)进行衍生化反应生成衍生物.实验采用Diamonsil C18(2)(迪马,150mm×4.6mm,3μm)色谱柱,以水-有机溶剂(乙腈-甲醇,体积比为6∶4)作为流动相,流速为0.5mL/min,用梯度洗脱程序方法同时分离5种多胺衍生物,衍生物的检测波长为362nm.通过考察方法的线性范围、稳定性、检测限和回收率表明:本方法具有样品处理简单、灵敏度高、速度快的优点,可用于啤酒中多胺含量的测定.A high performale liquid chromatography method was developed for the determination of polyamines in beer. The polyamines were derivatived by 1-（5-fluoro-2, 4-dinitrophenly）-4-methlypiperazine （PPZ） in alkaline condition and then separated on a Diamonsil C18 （2） （Dikma, 150×mm 4.6mm×3μm） column. The effect of mobile phase composition was investigated at detection wavelength of 362 nm. The result indicated that the optimum separation was obtained by using water： （acetonitrile ： methanol= 6：4, v/v） as the mobile phase with gradient elution （flow rate of 0.5 mL/min）. The linear range, stability, detection limit and recovery were examined. The method was simple, sensitive and rapid. The proposed method has been applied to the determination of polyamines in beer.

关 键 词：高效液相色谱 多胺 1-(5-氟-2 4-二硝基苯基)-4-甲基哌嗪 

分 类 号：O656.31[理学—分析化学]