安徽道地药材断血流的愈伤组织诱导条件研究  

Research on the Condition of Calls Induction of Genuine Clinopodii polycephali of Anhui

作　　者：陈宇 王芳 李耀亭[2] 

机构地区：[1]六安市西山生物技术有限公司,安徽六安237005 [2]皖西学院植物细胞工程安徽省工程技术研究中心,安徽六安237012

出　　处：《安徽农业科学》2014年第33期11667-11668,共2页Journal of Anhui Agricultural Sciences

基　　金：安徽省科技攻关计划项目(12010402083)

摘　　要：[目的]研究不同基础培养基及添加的植物生长调节剂浓度对断血流愈伤组织的诱导效果。[方法]通过配制不同的培养基,选择适宜诱导断血流愈伤组织的培养基;然后选用适宜的培养基,研究不同浓度的6-BA及NAA对断血流愈伤组织诱导的影响。[结果]MS、N6、B5、RM、Miller 5种基本培养基中,适合断血流愈伤组织诱导的基本培养基为MS;研究不同的生长素NAA及细胞分裂素6-BA浓度时发现,NAA为0.1 mg/L和6-BA为1.0 mg/L时,断血流组培效果较好。[结论]断血流组培效果最好的培养基是MS培养基中NAA为0.1 mg/L和6-BA为1.0 mg/L。[Objective] The research aimed to study the effect of the media types and the concentration of plant growth regulator on the callus induction of Clinopodii polycephali.[Method] Choose the suitable culture medium from the preparation of different kinds of medium.In the appropriate culture medium cases,the effect of the different concentration of 6-BA and NAA on inducing callus of Clinopodii polycephali were studied.[Result] MS was best to induce callus of Clinopodii polycephali among MS,N6,B5,RM,Miller.The research of the different concentrations of auxin NAA and cytokinin 6-BA found that when NAA was 0.1 mg/L and 6-BA was 1.0 mg/L,the effect on inducing callus of Clinopodii polycephali was better.[Conclusion] The best suitable medium was MS with NAA 0.1 mg/L and 6-BA 1.0 mg/L.

关 键 词：断血流 培养基 植物生长调节剂 诱导 愈伤组织 

分 类 号：S567[农业科学—中草药栽培]