伤科黄水对兔牵拉成骨区新骨生成的影响  

Effect of the traumatology yellow water on new bone formation of distraction osteogenesis zone in rabbit

作　　者：蔡立雄[1] 杨海韵[1] 孙丙银[2] 温建强[1] 吴峰[1] 吴玢[2] 严加取 

机构地区：[1]广州省佛山市中医院,广东佛山528000 [2]广州中医药大学,广东广州510006

出　　处：《中医正骨》2014年第10期12-15,共4页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：广东省中医药管理局科研项目(20130157)

摘　　要：目的:探讨伤科黄水对兔牵拉成骨区新骨生成的影响及作用机制。方法:对24只新西兰白兔左侧股骨进行牵拉成骨手术,手术成功后将所有兔子随机分为2组,每组12只。实验组手术切口处及克氏针针孔处外敷伤科黄水纱布,对照组切口处及克氏针针孔处外敷酒精纱布。术后通过肉眼观察、血液学检查及X线检查,比较2组动物的切口和新骨生成情况。结果:1肉眼观察。术后3 d,实验组动物手术切口表面及克氏针针孔处色红、干燥、少许结痂、肌肉组织轻度肿胀;术后5 d,切口表面干燥,远端克氏针针孔处肤色淡红;术后7 d,切口处有大量皮毛生长,切口已愈合,针孔处无感染迹象;术后10 d,切口完全愈合,皮毛生长接近正常。术后3 d,对照组动物切口色红、较湿润,远端有少许渗液,针孔处未见渗液,肌肉组织明显肿胀;术后5 d,切口皮肤色红、轻度湿润、肿胀,有少许结痂及皮毛生长;术后7 d,远端针孔处有少许渗液,肌肉组织轻度肿胀,有中等量皮毛生长;术后10 d,切口结痂愈合,无明显肿胀,表面干燥,有中等量皮毛生长。实验组动物比对照组创面愈合快[(7.5±0.6)d,(8.3±1.2)d,t=-2.066,P=0.025]。2血液学检查。术后5 d,实验组肿瘤坏死因子α含量和碱性成纤维细胞生长因子含量均高于对照组[(2.3±0.8)ng·mL-1,(1.8±0.5)ng·mL-1,t=1.836,P=0.040;(125.2±4.6)ng·L-1,(121.3±3.8)ng·L-1,t=2.264,P=0.017];术后7 d,实验组白细胞计数、中心粒细胞计数和百分比、淋巴细胞计数和百分比均低于对照组[(7.3±0.3)个·mL-1,(8.7±1.7)个·mL-1,t=-2.809,P=0.001;(4.0±0.9)个·mL-1,(5.1±1.6)个·mL-1,t=2.076,P=0.025;(54.8±1.7)%,(57.1±3.9)%,t=-1.873,P=0.037;(2.5±0.5)个·mL-1,(3.1±0.9)个·mL-1,t=2.019,P=0.028;(23.8±1.6)%,(25.3±1.4)%,t=2.444,P=0.011]。3X线检查。术后4周时的X线片示,2组动物牵拉成骨区均可见灰色低密度影,有大量新骨形成,骨皮质尚未连续;实验组牵拉成骨区骨小Objective：To explore the effect and its mechanism of action of the traumatology yellow water on new bone formation of dis- traction osteogenesis （DO）zone in rabbit. Methods：Twenty-four New Zealand white rabbits were obtained for DO surgery in the left femurs, after the surgery all the rabbits enrolled in the study were randomly divided into 2 groups, 12 cases in each group. The traumatology yellow water gauze（ experimental group）and alcohol gauze （control group）were applied to the surgical incisions and Kirschner wires pinholes of rabbits. After the surgery, the rabbits were compared between the 2 groups in incisions and new bone formation by the means of macroscopic observation, hematological examination and X-ray examination. Results：Three days after surgery, the surgical incisions and Kirschner wires pinholes of the rabbits in experimental group showed red, dry, a little callus and mild swelling. Five days after surgery, the incision surface were dry and the color of skin around distal Kirschner wires pinholes were reddish. Seven days after surgery, a lot of newborn fur were found around the incisions and the incisions had healed up and there was no evidence of infection in pinholes. Ten days after surgery, the incisions healed completely and the growth of fur approach normal. Three days after surgery, the surgical incisions of the rabbits in control group were red and wet and there was a few seepage in the distal end of incisions. No seepage was found in pinholes and obvious swelling was found in muscular tissue. Five days after surgery, the incisions skin were red, mild wet and swollen and a little callus and fur were found around it. Seven days after surgery,there were a few seepage in distal pinholes and the muscular tissue was mild swollen and the amount of fur was moderate. Ten days after surgery, the incisions healed without obvious swelling, The surface of incision was dry and moderate amount of fur was found around the incision. The incisions healing time of rabbits in experimental g

关 键 词：骨生成 牵张 伤科黄水 动物实验 

分 类 号：R285.5[医药卫生—中药学]