基于分子技术的土壤微生物多样性研究进展  

Research progress on soil microbial diversity based on molecular techniques

作　　者：谭益民[1,2] 何苑皞[1,2] 郭文平 

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室,湖南长沙410004 [2]中南林业科技大学林学院,湖南长沙410004 [3]湖南省攸县黄丰桥国有林场,湖南株洲412307

出　　处：《中南林业科技大学学报》2014年第10期1-9,共9页Journal of Central South University of Forestry & Technology

基　　金：林业公益性行业科研专项项目(201004014);中南林业科技大学研究生科技创新基金资助项目(2010bx02)

摘　　要：土壤微生物多样性是维系土壤功能的重要因素,但是由于传统的研究方法存在一定的局限性,使得过去研究其多样性及群落结构成为一个难题。随着分子生物学方法的应用,现在可以同时对可培养微生物和不可培养微生物进行研究,解决了传统培养方法存在的问题。综述了土壤微生物多样性的分子生物学研究方法,对比了各方法的优缺点,为选择合适的方法进行土壤微生物多样性研究提供了参考。Microbial diversity within the soil is crucial to many functions but the traditional research methods had some limitations thus making the studies of the diversity and community structure to be difficult problems. With the application of molecular biological methods, it is now possible to detect both culture-able and un-culture-able microbial species and solve the problems of traditional methods. This review was focused on the molecular methods of soil microbial diversity and the advantages and disadvantages of these methods were compared, thus providing reference for appropriate method for microbial diversity research.

关 键 词：土壤微生物多样性 分子生物学技术 研究进展 

分 类 号：S718[农业科学—林学]