3,5-二硝基水杨酸分光光度法测定羟乙基淀粉还原端条件研究  

Studies on Determination Conditions for Reducing End of Hydroxyethyl Starch by DNS Spectrophotometry

作　　者：刘亚萍[1] 张燕[2] 徐红[1] 周星彤[2] 杨钊[2] 

机构地区：[1]青岛大学医学院药学系,山东青岛266021 [2]青岛市药品检验所

出　　处：《中国药师》2014年第10期1772-1774,共3页China Pharmacist

摘　　要：目的:研究3,5-二硝基水杨酸(DNS)法测定羟乙基淀粉还原端的条件。方法:以葡萄糖做标准曲线来测定羟乙基淀粉还原端的含量,研究DNS试剂的用量、加热温度、加热时间及反应后放置时间对测定结果的影响。结果:DNS法测定羟乙基淀粉的最佳反应条件是:DNS试剂用量0.8 ml,反应温度85℃,反应时间5 min,显色定容后于540 nm的波长处测定吸光度。结论:本方法操作简单、准确,重复性好,可用于羟乙基淀粉还原端的测定。Objective： To explore the determination conditions for reducing end of hydroxyethyl starch by DNS spectrophotometry. Methods： The reducing end of hydroxyethyl starch was determined using the standard curve of glucose solutions. The effects of DNS reagent with different volume, heating temperature, heating time and standing time after reaction on the determination were investiga- ted. Results： The optimal determination conditions were as follows ： the DNS volume was 0.8 ml, the reaction temperature was 85 ℃, the reaction time was 5 minutes, and the colored solution was determined at the wavelength of 540 nm. Conclusion ： The method is simple and accurate with good reproducibility, which can be used to determine reducing end of hydroxyethyl starch.

关 键 词：3  5-二硝基水杨酸 还原糖 羟乙基淀粉 紫外光度法 

分 类 号：R917[医药卫生—药物分析学]