HPLC法测定何首乌中二苯乙烯苷的含量  

Determination of Stilbence Glucoside in Polygonum Multiflorum by HPLC

作　　者：兰婷[1] 王宝龙[1] 李明华[1] 李穆琼[2] 何炜[1] 

机构地区：[1]第四军医大学药学系化学教研室,西安710032 [2]第四军医大学药学系药物化学教研室,西安710032

出　　处：《中国药师》2014年第10期1774-1775,共2页China Pharmacist

基　　金：陕西省自然科学基础研究计划重点项目(编号:2013JM4005)

摘　　要：目的：建立以高效液相法测定何首乌水提液中二苯乙烯苷含量的方法。方法：色谱柱为Agilent Eclipse Plus C18（100 mm ×4.6 mm,3.5μm）,柱温为25℃,流动相为乙腈∶水=18∶82,流速为1.0 ml·min^-1,检测波长为320 nm,进样量为8μl。结果：二苯乙烯苷在38~237.5μg·ml^-1（r=0.9994）范围内线性关系良好,回收率为98.32%,RSD=1.59%。结论：本法简单、灵敏、专属性强,可用于何首乌中二苯乙烯苷的含量测定。Objective：To determine the content of stilbene glucoside in polygonum multiflorum by HPLC. Methods： An Agilent Eclipse Plus C18（100 mm ×4.6 mm,3.5 μm）column was adopted with acetonitrile-water（18∶82） as the mobile phase at a flow rate of 1. 0 ml·min^-1 . The detection wavelength was set at 320nm and the injection volume was 8 μl. Results：The linear range of stilbene glucoside was 38-237. 5 μg·ml^-1（r=0. 999 4）with the average recovery of 98. 32%（RSD=1. 59%）. Conclusion： The method is simple, sensitive and specific in the content determination of stilbene glucoside in polygonum multiflorum.

关 键 词：二苯乙烯苷 何首乌 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]