癌痛消颗粒质量标准研究  

Study on Quality Standard for Aitongxiao Granules

作　　者：农毅清 蒋林 谭安蔷[2] 

机构地区：[1]南宁食品药品检验所,南宁530001 [2]广西中医学院制药厂

出　　处：《中国药师》2014年第11期1859-1861,共3页China Pharmacist

摘　　要：目的：建立癌痛消颗粒的质量标准。方法：采用薄层色谱法对处方中白花蛇舌草、半枝莲、黄芪、赤芍4味中药进行定性鉴别,采用HPLC法测定半枝莲中野黄芩苷的含量,以Kromasial C18柱（250 mm×4.6 mm,5μm）为色谱柱,流动相为甲醇-3﹪冰醋酸溶液（33∶67）,流速为1.0 ml·min^-1,检测波长为335 nm,柱温为25℃。结果：各供试品色谱中,在与各自对照品色谱相同的位置上显相同颜色的斑点,且阴性对照无干扰。HPLC法测定野黄芩苷在2.18-10.90μg·ml^-1范围内线性关系良好（r=0.999 9）,平均加样回收率为96.65%,RSD=1.45%（n=6）。结论：本方法简便、准确、重现性好,适用于癌痛消颗粒的质量控制。Objective：To establish the quality standard for Aitongxiao granules. Methods：TLC was used to identify Herba Hedyotis Diffusa, Herba Scutell Barbatae, Radix Astragali and Radix Paeoniae Rubra in Aitongxiao granules, and the content of scutellarin was determined by HPLC. The stationary phase was a Kromasial C18 column （250 mm × 4. 6 mm,5μm）, the mobile phase was methanol-3% acetic acid （33∶67）, the flow rate was 1. 0 ml·min-1, the detection wavelength was at 335 nm, and the temperature was 25℃. Results：The characteristic TLC spots of the granule samples were the same as those of the standard samples without any inter-ference from the negative control. Scutellarin had a good linear relationship within the range of 2.18-10.90μg·ml-1（r=0.999 9）, and the average recovery was 96. 65% with RSD of 1. 45﹪（n=6）. Conclusion：The method is simple, accurate, reliable and reproducible, which could be used in the quality control of Aitongxiao granules.

关 键 词：癌痛消颗粒 薄层色谱 高效液相色谱法 

分 类 号：TQ460.72[医药卫生—药物分析学]