宿州市454份样本HIV抗体确证实验结果及带型分析  

Confirmatory experiment results and banding pattern analysis of 454 HIV antibody samples in Suzhou

作　　者：胡雪影[1] 张芳[1] 邱山[1] 杨菊[1] 缪礼峰 

机构地区：[1]宿州市疾病预防控制中心,安徽宿州234000 [2]安徽省疾病预防控制中心,安徽合肥230601

出　　处：《安徽预防医学杂志》2014年第5期329-331,共3页Anhui Journal of Preventive Medicine

摘　　要：目的对2009-2013年宿州市454份人类免疫缺陷病毒（HIV）抗体待确证样本进行免疫印迹（WB）条带分析,增强对WB试验结果的分析判断能力。方法采用WB试验对454份宿州市辖区HIV抗体待确证样本进行检测。结果 454例HIV抗体待确证样本中,WB试验阳性235份,占51.76%;95份为不确定,占20.93%;124份为阴性,占27.31%。WB带型分布：7条带及以上222份,占94.47%;抗env基因编码蛋白的条带较多,gp160、gp120分别为98.72%和98.32%;除p24外的抗gag基因编码蛋白的条带最少;在95份HIV确证不确定样品和124份HIV确证阴性样品中,采供血机构送检的样本比例均最高,分别是66.32%、79.84%。结论两种试剂复检仍存在一定的假阳性,WB确证试验存在不确定结果,应引入病毒载量或核酸检测技术缩短HIV抗体检测中的不确定样本的确诊时间。Objective To perform WB banding pattern analysis on 454 HIV antibody to be confirmed samples in Suzhou from 2009 -2013, and enhance the ability of analyzing and judging the WB experimental results. Methods 454 HIV antibody positive samples detected by ELISA were confirmed by immunoblotting test （ WB ）. Results There were 235 WB confirmed positive cases, accounting for 51.76%, 95 cases were uncertain, accounting for 20.93%, and 124 cases were negative, accounting for 27.31%. From the distribution of WB banding pattern, there were 222 cases with 7 strips and above, accounting for 94.47%. The positive rate of protein antibody encoded by env was high, with gp160 and gp120 were 98. 72% and 98.32%, respectively. The positive rate of Pol antibody was lower. The positive rate of gag antibody was the lowest except io24. In 95 uncertain samples and 124 HIV negative samples,the blood transfusion services had the highest proportion, which were 66.32% and 79.84%, respectively. Conclusion There are still some false positive results rechecked by two kinds of reagent, and WB test has indeterminate result. In order to reduce the confirming time of uncertain samples, we should introduce viral load or nucleic acid detection technology.

关 键 词：人类免疫缺陷病毒 确证试验 确证条带分析 

分 类 号：R759[医药卫生—皮肤病学与性病学]