血清游离DNA检测在系统性红斑狼疮患者的应用  

Application of detecting serum cell-free DNA in patients with systemic lupus erythematosus

作　　者：施维[1] 吴信华[1] 钱捷[2] 戚菁[1] 张玉泉[3] 张建林[3] 鞠少卿[1] 

机构地区：[1]南通大学附属医院外科综合实验室,江苏省226001 [2]南通大学附属医院风湿科,江苏省226001 [3]南通大学附属医院妇产科,江苏省226001

出　　处：《江苏医药》2014年第21期2537-2540,共4页Jiangsu Medical Journal

基　　金：江苏省六大人才高峰项目(2012-WS-034);南通市科技项目(HS2016056);南通大学校级自然科研项目(11Z014)

摘　　要：目的探讨检测系统性红斑狼疮(SLE)患者血清游离DNA的临床意义。方法采用分支DNA(bDNA)技术检测69例SLE患者(SLE组)、26例RA患者(RA组)和41例健康人(C组)血清游离DNA浓度,分析SLE患者抗dsDNA抗体、抗SmD1抗体、补体、24-h尿蛋白定量、C反应蛋白、血沉和SLE疾病活动指数(SLEDAI)与血清游离DNA浓度的相关性。结果与C组和RA组相比,SLE组患者血清游离DNA水平增高(P<0.01)。SLE组抗SmD1抗体阳性患者的血清游离DNA水平高于抗SmD1抗体阴性者(P<0.05)。SLE患者血清游离DNA浓度与血清抗dsDNA抗体、SLEDAI、血沉呈负相关(P<0.05),而与24-h尿蛋白定量、补体、C反应蛋白无明显相关性(P>0.05)。结论血清游离DNA检测可用于评估SLE病情进展。Objective To explore the significance of detecting serum cell-free DNA in the patients with systemic lupus erythematosus（SLE）.Methods Serum cell-free DNA was detected in SLE patients（group SLE,69cases）,patients with rheumatoid arthritis（group RA,26cases）and healthy people（group C,41cases）.The correlation between serum cell-free DNA and anti-double strand DNA（anti-dsDNA）antibody,anti-SmD1 antibody,24-h urine protein,complements and Creactive protein（CRP）,erythrocyte sedimentation rate（ESR）and SLE disease activity index（SLEDAI）was analyzed.Results Compared with groups of C and RA,serum cell-free DNA level in group SLE was significantly higher（P〈0.01）,which was higher in SLE patients with anti-SmD1 antibody positive than those with it negative（P〈0.05）.Serum level of cell-free DNA was negatively correlated with anti-dsDNA,SLEDAI and ESR（P〈0.05）.There was no significant correlation between serum level of cell-free DNA and 24-h urine protein,complements and CRP（P〈0.05）.ConclusionDetection of serum cell-free DNA may be used to evaluate the progression of SLE patients.

关 键 词：系统性红斑狼疮 游离DNA 

分 类 号：R593[医药卫生—内科学]