管材和流速对供水管道生物膜形成的影响  

Interaction of flow velocities and pipe materials on biofilm formation

作　　者：祝泽兵[1,2] 吴晨光[1,2] 钟丹[1,2] 袁一星[1,2] 单莉莉[1,2] 张杰[1,2] 袁媛[1,2] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090 [2]哈尔滨工业大学市政环境工程学院,哈尔滨150090

出　　处：《哈尔滨工业大学学报》2014年第10期31-36,共6页Journal of Harbin Institute of Technology

基　　金：国家自然科学基金资助项目(青年)(51108123);城市水资源与水环境国家重点实验室(哈尔滨工业大学)自主课题(2014TS08)

摘　　要：针对供水管网中不断变换的管材和水力条件,运用异养菌计数和PCR-DGGE技术研究了特定余氯质量浓度下管材和流速对水质和管道生物膜形成的影响.结果表明:管材对主体水余氯、TOC、细菌总数和生物膜群落结构影响均较大,而流速只对主体水余氯和生物膜细菌总数影响较大;同一时期,PE管道附着细菌总数显著大于不锈钢;同类管材中0.2和0.4 m/s流速下生长的生物膜细菌总数差异不显著,但均显著大于0.8 m/s流速下生长的生物膜细菌总数;生物膜成熟时鞘氨醇单胞菌属(Sphingomonas)和芽孢杆菌属(Bacillus)占优势且广泛存在,而某些疏水性细菌则较难在不锈钢管材上聚集,特别是在流速增大时更难聚集.The flow velocities and pipe materials may be variable in drinking water distribution system, and their interaction on the water quality and biofilm formation under a certain chlorine level have been investigated. Heterotrophic plate counts and denaturing gradient gel electrophoresis were employed to analyze biofilm formation, and the results showed that the chlorine, TOC of bulk water, total bacteria counts and microbial community structure were all varied in pipe materials, while flow velocities only affected the chlorine of bulk water and biofilm biomass. In the same time, PE pipes attached significantly greater biofilm biomass than stainless steel. The biofilm biomass grown under flow velocities 0. 2 and 0.4 m/s were significantly greater than that under 0. 8 m/s, but the biofilm growth between 0. 2 and 0. 4 m/s were not significant. In the mature biofilm microbial community, Sphingomonas sp. and Bacillus sp. were dominant in all biofilms, while the hydrophobic bacteria were difficult to grow on the stainless steel pipe, especially under high flow velocities.

关 键 词：管材 流速 生物膜 微生物群落结构 

分 类 号：TU991.21[建筑科学—市政工程]