污泥发酵液对A^2O脱氮除磷和微生物的影响  

The effect of sludge fermentation liquid on nutrient removal performances and microbial community structure in A^2O process

作　　者：刘亚利[1] 袁一星[1] 李欣[1] 詹技灵 康晓荣[1] 董林沛[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,哈尔滨150090 [2]大连迈克环境科技有限公司,辽宁大连116000

出　　处：《哈尔滨工业大学学报》2014年第10期42-46,共5页Journal of Harbin Institute of Technology

基　　金：国家高技术研究发展计划(863计划)资助项目(2012AA063503-02)

摘　　要：为研究剩余污泥发酵液作碳源对微生物群落结构的影响,将发酵液与市政污水按流量比1∶35回用于厌氧-缺氧-好氧反应器,在室温下运行90 d.聚类分析表明,发酵液明显改变了微生物群落结构,5~30 d和45~90 d的微生物属于不同的聚集区;微生物多样性分析表明,发酵液使Shannon-Wiener指数从2.6升高到3.1,系统运行稳定性增强;PCR-DGGE分析表明,发酵液对微生物群落具有一定的选择性,氨氧化菌Nitrosomonas sp.、硝化菌Betaproteobacteria和Nitrospira sp.、反硝化菌Comamonas sp.和聚磷菌Gammaproteobacteria得到富集,TN和TP去除率从64.5%和52.4%提高到84.7%和94.3%.To analyze the effect of sludge fermentation liquid, using as internal carbon source, on microbial community structure in anaerobic-anoxic-aerobic process, three-month-long operational experiment was conducted at flow ratio of fermentation liquid and domestic wastewater 1 ： 35 at room temperature. The clustering analysis indicated that the microbial community structure was changed significantly by fermentation liquid, and the microbes of 5-30 d and 45-90 d had quite different homology. The microbial diversity analysis demonstrated that the Shannon-Wiener index increased from 2.6 to 3.1, resulting in the enhancement of operational stability. Meanwhile, fermentation liquid appeared to be selective for ammonia-oxidizing bacteria Nitrosomonas sp., nitrifying bacteria Betaproteobacteria and Nitrospira sp., denitrifying bacteria Comamonas sp. and phosphorus-accumulating bacteria Gammaproteobacteria, which led to the TN and TP removal efficiency improved from 64. 5% and 52.4% to 84. 7% and 94. 3%, respectively.

关 键 词：剩余污泥 脱氮除磷 碳源 生物群落 聚合酶链式反应-变性梯度凝胶电泳技术 

分 类 号：TU992.3[建筑科学—市政工程]