离子液体对毛细管电泳分离美托洛尔和普萘洛尔对映体的影响研究  

Effect of ionic liquids on enantioseparation of mixed metoprolol and propranolol by capillary electrophoresis

作　　者：王钰[1] 杨思文[1] 代语林 邓宁[1] 王燕[1] 何建波[1] 朱燕舞[1] 

机构地区：[1]合肥工业大学化学与化工学院,合肥230009

出　　处：《药物分析杂志》2014年第11期2091-2097,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(No.21102030手性离子液体键合整体柱毛细管电色谱新技术及其在手性拆分中的应用)资助

摘　　要：目的:以离子液体1-丁基-3-甲基咪唑双三氟甲磺酰亚胺盐(BMIMNTF2)和羧甲基-β-环糊精(CM-β-CD)共同作为手性选择剂,建立分离美托洛尔和普萘洛尔混合对映体毛细管电泳方法。方法:采用未涂层毛细管(有效长度42cm,内径50μm),考察了离子液体的类型及浓度、缓冲溶液的浓度、缓冲溶液p H、分离电压对手性拆分的影响。结果:毛细管电泳同时分离美托洛尔对映体和普萘洛尔对映体的最佳电泳条件:以5mmol·L#1BMIMNTF2和5 mmol·L#1CM-β-CD共同作为手性选择剂,缓冲溶液为73 mmol·L#1pH 6.0的Tris-H3PO4溶液,分离电压为20 kV,检测波长为230 nm,高差10 cm进样15s,室温下进行。美托洛尔和普萘洛尔混合对映体在18 min内达到基线分离。结论:BMIMNTF2与CM-β-CD的协同作用不仅可以改善美托洛尔和普萘洛尔之间的分离,而且可以提高美托洛尔对映体和普萘洛尔对映体手性分离的选择性。Objective： To establish a capillary electrophoresis method for chiral separation of metoprolol enantiomers and propranolol enantiomers,with 1- butyl- 3- methylimidazolium bis（ trifluoromethylsulfonyl） imide（ BMIMNTF2）and carboxymethyl- β- cyclodextrin（ CM- β- CD） as the chiral selectors. Methods： The analysis was performed on a fused- silica capillary of 50 μm internal diameter and 42 cm efficient length. The effects of ionic liquid types and concentrations,the concentration of the buffer solution,buffer p H and separation voltage on chiral separation were investigated. Results： The best electrophoresis conditions of simultaneous chiral separation of metoprolol enantiomers and propranolol enantiomers were as follows. The 5 mmol·L^-1 BMIMNTF2and the 5 mmol·L^-1 CM- β- CD were taken as the chiral selectors,the buffer solution was 73 mmol·L^-1 Tris- H3PO4 at p H 6. 0,the applied voltage was 20 kV,the detection wavelength was 230 nm and the sampling time was 15 s. The baseline separation was achieved for metoprolol enantiomers and propranolol enantiomers within 18 min. Conclusion： The research proves that CM- β- CD and BMIMNTF2 as chiral selectors can improve the separation and the enantioseparation selectivity of metoprolol and propranolol.

关 键 词：离子液体手性选择剂 丁基-甲基咪唑双三氟甲磺酰亚胺盐(BMIMNTF2) 羧甲基-β-环糊精(CM-β-CD) 毛细管电泳 对映体分离 美托洛尔 普萘洛尔 

分 类 号：R917[医药卫生—药物分析学]