ICP-MS法分析新促排灵对铅中毒小鼠的促排作用  

Effect of the chelator Zn-DTPA on the excretion of lead in lead intoxication mice detected with ICP-MS

作　　者：陈立[1] 陆开智 周琪[1] 汪琼[1] 曾瑜亮 殷红军[1] 何轩辉[1] 田瑛[1] 董俊兴[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]军事医学科学院野战输血研究所,北京100850

出　　处：《药学学报》2014年第11期1588-1592,共5页Acta Pharmaceutica Sinica

基　　金：国家"重大新药创制"科技重大专项(2009ZXJ09001-025)

摘　　要：应用电感偶合等离子体质谱(ICP-MS)测定生物样本中铅的浓度,考察核素促排药新促排灵对铅中毒小鼠的促排作用。小鼠一次性腹腔注射乙酸铅溶液,每只染铅1 mg,建立急性铅中毒模型,染铅后4 h腹腔注射新促排灵(Zn-DTPA),连续给药5天。同时设正常对照组、染铅模型组、新促排灵与促排灵(Ca-DTPA)联用组。每天收集尿液,在实验的第2、4和6天分别处死部分小鼠,取全血、双侧股骨、脑,消解处理后,用ICP-MS测定铅含量。结果表明Zn-DTPA能显著增加尿铅的排出,降低血液、股骨和脑组织中的铅含量。To study the lead excretion effect of the chelator Zn-DTPA on the lead intoxication mice,inductively coupled plasma mass spectrometry（ICP-MS） was applied to detect the lead content of biological samples. The acute lead intoxication mice model was established by injecting lead acetate intraperitoneally with the dose of 1 mg. Zn-DTPA was administered intraperitoneally to mice once daily for five consecutive days 4 h after intoxication. Control group,model group,combination of Zn-DTPA and Ca-DTPA group were evaluated at the same time. The urine was collected every day. The mice were sacrificed in batches in the 2rd,4th,6th day. Biological samples including urine,whole blood,femur and brain were prepared and nitrated. Lead concentration was detected by ICP-MS. The result showed that Zn-DTPA could increase lead content in urine markedly and reduce lead content in blood,femur and brain.

关 键 词：新促排灵 电感偶合等离子体质谱 铅中毒 排铅 

分 类 号：R944[医药卫生—药剂学] R917[医药卫生—药学]