利多卡因联合丙胺卡因对金黄色葡萄球菌抑菌效果的评价  

Evaluation of antibacterial effect of lidocaine /prilocaine on Staphylococcus aureus

作　　者：顾飞飞[1] 蒋婕[2] 项瑾[2] 肖淑珍[1] 赵声远[1] 张骥[3] 倪语星[1] 韩立中[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院临床微生物科,上海200025 [2]上海交通大学医学院瑞金临床医学院检验系,上海200025 [3]上海市普陀区人民医院检验科,上海200060

出　　处：《上海交通大学学报（医学版）》2014年第10期1485-1488,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：卫生公益性行业科研专项(201002021);上海市普陀区卫生系统自主创新科研资助项目(普KW11204)~~

摘　　要：目的评价利多卡因联合丙胺卡因（利多卡因/丙胺卡因）对金黄色葡萄球菌的抑菌效果。方法收集皮肤及软组织感染分离的金黄色葡萄球菌共126株。采用纸片扩散法检测青霉素、头孢西丁、庆大霉素等18种抗生素的耐药性,根据头孢西丁药物敏感试验结果结合mecA基因筛选耐甲氧西林金黄色葡萄球菌（MRSA）,采用E-test检测万古霉素最低抑菌浓度（MIC）。采用琼脂稀释法测定利多卡因/丙胺卡因对金黄色葡萄球菌的MIC。结果 126株金黄色葡萄球菌中23株为MRSA,耐药率较高的为青霉素和红霉素,分别是86.5%和46.8%;未检出对替考拉宁、万古霉素、利奈唑胺和奎奴普丁/达福普汀耐药的菌株。利多卡因/丙胺卡因对金黄色葡萄球菌的MIC范围为（1/1～2/2）mg/mL,MIC50和MIC90均为（2/2）mg/mL。结论利多卡因/丙胺卡因对皮肤及软组织感染分离的金黄色葡萄球菌具有明显的抑菌效果,在作为止痛、止痒药物运用于皮肤病患者时,可有效预防和抑制金黄色葡萄球菌的感染。Objective To evaluate the antibacterial effect of lidocaine/prilocaine on Staphylococcus aureus（S. aureus）. Methods One hundred and twenty-six S. aureus isolates were collected from patients with skin and soft tissue infection（ SSTI）.The drug resistance of S. aureus towards 18 antibiotics, including penicillin,cefoxitin, and gentamicin, were measured by the disk diffusion method. Methicillin-resistant S. aureus（MRSA）was screened based on the result of antimicrobial susceptibility test of cefoxitin and mecA gene.The minimum inhibitory concentration（MIC） of vancomycin was detected by E-test.The agar dilution method was adopted to detect the MIC of lidocaine /prilocaine. Results Twenty-three of 126 S. aureus isolates were MRSA.The resistance rates of penicillin and erythromycin were higher and were 86. 5% and 46. 8%, respectively. No isolates were resistant to teicoplanin, vancomycin, linezolid, and quinupristin /dalfopristin.The MIC range of S.aureus lidocaine /prilocaine towards was（ 1 /1 ～ 2 /2） mg /mL and M IC50 and MIC90 were both（ 2 /2） mg /mL.Conclusion Lidocaine /prilocaine shows significant antibacterial effect on S. aureus isolated from patients with skin and soft tissue infections.They can stop pain and itch for patients with skin diseases and effectively prevent and inhibit S. aureus infections.

关 键 词：利多卡因 丙胺卡因 金黄色葡萄球菌 皮肤及软组织感染 抑菌效果 

分 类 号：R96[医药卫生—药理学]