“看似正常者”外周血单个核细胞炎症因子的表达变化  

Pro- inflammatory and anti- inflammatory cytokine responses of peripheral blood mononuclear cells in apparently healthy subjects

作　　者：郜珊珊[1] 舒珊[1] 王丽君[1] 周娟[1] 袁祖贻[1] 

机构地区：[1]西安交通大学第一附属医院心内科,陕西西安710061

出　　处：《南方医科大学学报》2014年第11期1589-1593,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30871043)~~

摘　　要：目的观察"看似正常者"体内炎症因子表达是否已经发生变化。方法按照JUPITER研究的入选标准和排除标准,从西安交通大学第一附属医院体检中心选取正常对照和"看似正常者"各14例,采集晨空腹静脉血5 ml后,密度梯度离心法提取外周血单个核细胞,提取RNA并进行real-time PCR检测各炎症因子的表达。同时留取血浆行IL-6和TNF-α的ELISA检测。结果 "看似正常者"促炎因子TNF-αmRNA表达增高,血浆浓度升高;促炎因子IL-6 mRNA表达及血浆浓度,MCP-1 mRNA表达仅表现出轻微升高趋势;MCPIP由MCP-1诱导产生,mRNA表达升高;抗炎因子IL-10 mRNA表达降低。结论 "看似正常者"体内炎症因子表达已发生变化,促炎因子表达相对升高,抗炎因子表达相对降低。Objective To examine the changes in the expression of pro- inflammatory and anti- inflammatory cytokines in the peripheral blood mononuclear cells（PBMCs） and elucidate the inflammatory status of apparently healthy subjects. Methods Peripheral blood samples（5 ml） were collected after fasting for more than 8 h from 14 healthy control subjects and 14 apparently healthy subjects with elevated serum high-sensitivity CRP（hsCRP） level（≥2.0 mg/L）. PBMCs were separated by Ficoll density gradient centrifugation and the total RNA was extracted for real- time quantitative PCR analysis of the inflammatory cytokines, and plasma was separated for ELISA analysis of interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） expressions. Results The gene expressions of TNF-α and MCPIP were significantly increased in PBMCs of apparently healthy subjects, while IL-6 and MCP-1 only showed slight elevations; IL-10 expression in PBMCs decreased significantly in apparently healthy subjects as compared to that in the control group. The results of ELISA showed significantly elevated TNF-α level without significant changes of plasma IL-6 level in apparently healthy subjects. Conclusion In apparently healthy subjects with normal lipid levels, chronic low- grade inflammation has occurred shown by elevated expressions of the proinflammatory cytokines and lowered expressions of the anti-inflammatory cytokines.

关 键 词：C-反应蛋白 “看似正常者” 炎症 细胞因子 

分 类 号：R541.4[医药卫生—心血管疾病]