5-氨基酮戊酸介导的光动力疗法预防兔耳瘢痕增生的初步探讨  

Prevention of hypertrophic scars by 5 -aminolaevulinic acid-mediated photodynamic therapy in a rabbit ear model

作　　者：胡丽[1] 李昕[2] 黄惠真 李伟[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,上海200011 [2]郑州大学第一附属医院皮肤科

出　　处：《中国美容整形外科杂志》2014年第11期696-699,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金资助项目（81071565）

摘　　要：目的：探讨5-氨基酮戊酸介导的光动力疗法抑制兔耳瘢痕增生的效果。方法建立兔耳增生性瘢痕模型后,将108个增生性瘢痕块随机分为4组：空白对照组、单纯激光组、单纯5-氨基酮戊酸组及5-氨基酮戊酸介导的光动力治疗组。5-氨基酮戊酸介导的光动力治疗组在局部注射5-氨基酮戊酸后5h行半导体激光照射,波长635nm,功率密度100mW/cm2,照射20min。治疗后观察3周瘢痕的生长情况,测量瘢痕厚度及红斑指数,取材后行HE染色,观察真皮层厚度的变化,计算各组瘢痕增生指数,Masson染色观察胶原纤维排列情况,TUNEL染色观察成纤维细胞凋亡情况。结果5-氨基酮戊酸介导的光动力治疗组瘢痕厚度与红斑指数较其他3组均降低（P〈0.05）；真皮层厚度明显变薄,瘢痕增生指数较其他3组明显降低（P〈0.05）；Masson染色显示,5-氨基酮戊酸介导的光动力治疗组真皮层内胶原纤维较其他3组减少,且排列整齐有序；TUNEL染色显示,5-氨基酮戊酸介导的光动力治疗组成纤维细胞凋亡数量较其他3组明显增多（P〈0.05）。结论应用5-氨基酮戊酸介导的光动力疗法可以预防兔耳瘢痕增生。Objective To investigate the effects of 5 - aminolaevulinic acid- mediated photodynamic therapy （ALA-PDT）on hypertrophic scars in a rabbit ear model.Methods The acute model of dermal hypertrophic scar was established in the rabbit ears.Totally 108 scar wounds were randomly divided into 4 groups：the blank control group,the laser treatment group,the ALA injection group and the ALA-PDT group.In the ALA-PDT group,5 h after intra-dermal injection of ALA,the scars were irradia-ted at 635 nm wavelength, 100 mW/cm2 for 20 min.Three weeks after treatment,the scars thickness and erythema index were measured,and the specimens were harvested for histological analysis by HE and Masson staining.Results The thickness,erythema index and the scar elevation index were signifi-cantly decreased while the number of apoptosis of fibroblasts increased in the ALA-PDT group com-pared to the other groups.Well organized collagen fiber was observed in the ALA-PDT group by Mas-son staining.Conclusion ALA-PDT could prevent the growth of hypertrophic scars in rabbit ears.

关 键 词：5-氨基酮戊酸 光动力疗法 增生性瘢痕 

分 类 号：R619.6[医药卫生—外科学]