Effects of Curcumin on Pain Threshold and on the Expression of Nuclear Factor κ B and CX3C Receptor 1 after Sciatic Nerve Chronic Constrictive Injury in Rats  

作　　者：曹红 郑晋伟 李佳佳 孟波 李军 葛仁山 

机构地区：[1]Department of Anesthesiology,The Second Affiliated Hospital of Wenzhou Medical College

出　　处：《Chinese Journal of Integrative Medicine》2014年第11期850-856,共7页中国结合医学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China(No.81073125);Natural Science Foundation of Zhejiang Province(No.Y2090252);International Cooperation Project of Wenzhou Science and Technology Bureau(No.H20070035)

摘　　要：Objective：To investigate the effects of curcumin on pain threshold and the expressions of nuclear factorκB（NF-κB）and CX3C chemokine receptor 1（CX3CR1）in spinal cord and dorsal root ganglion（DRG）of the rats with sciatic nerve chronic constrictive injury.Methods：One hundred and twenty male Sprague Dawley rats,weighing 220-250 g,were randomly divided into 4 groups.Sham surgery（sham）group：the sciatic nerves of rats were only made apart but not ligated;chronic constrictive injury（CCI）group：the sciatic nerves of rats were only ligated without any drug treatment;curcumin treated injury（Cur）model group：the rats were administrated with curcumin 100 mg/（kg·d）by intraperitoneal injection for 14 days after CCI;solvent control（SC）group：the rats were administrated with the solvent at the same dose for 14 days after CCI.Thermal withdrawal latency（TWL）and mechanical withdrawal threshold（MWT）of rats were respectively measured on pre-operative day 2 and postoperative day 1,3,5,7,10 and 14.The lumbar segment L4-5 of the spinal cord and the L4,L5 DRG was removed at post-operative day 3,7 and 14.The change of nuclear factorκB（NF-κB）p65 expression was detected by Western blotting while the expression of CX3CR1 was determined by immunohistochemical staining.Results：Compared with the sham group,the TWL and MWT of rats in the CCI group were significantly decreased on each post-operative day（P〈0.01）,which reached a nadir on the 3rd day after CCI,and the expressions of NF-κB p65and CX3CR1 were markedly increased in spinal cord dorsal horn and DRG.In the Cur group,the TWL of rats were significantly increased than those in the CCI group on post-operative day 7,10 and 14（P〈0.05）and MWT increased than those in the CCI group on post-operative day 10 and 14（P〈0.05）.In addition,the administration of curcumin significantly decreased the positive expressions of NF-κB p65 and CX3CR1 in spinal cord and DRG（P〈0.05）.Conclusion：Our study suggests that curcumin couObjective：To investigate the effects of curcumin on pain threshold and the expressions of nuclear factorκB（NF-κB）and CX3C chemokine receptor 1（CX3CR1）in spinal cord and dorsal root ganglion（DRG）of the rats with sciatic nerve chronic constrictive injury.Methods：One hundred and twenty male Sprague Dawley rats,weighing 220-250 g,were randomly divided into 4 groups.Sham surgery（sham）group：the sciatic nerves of rats were only made apart but not ligated;chronic constrictive injury（CCI）group：the sciatic nerves of rats were only ligated without any drug treatment;curcumin treated injury（Cur）model group：the rats were administrated with curcumin 100 mg/（kg·d）by intraperitoneal injection for 14 days after CCI;solvent control（SC）group：the rats were administrated with the solvent at the same dose for 14 days after CCI.Thermal withdrawal latency（TWL）and mechanical withdrawal threshold（MWT）of rats were respectively measured on pre-operative day 2 and postoperative day 1,3,5,7,10 and 14.The lumbar segment L4-5 of the spinal cord and the L4,L5 DRG was removed at post-operative day 3,7 and 14.The change of nuclear factorκB（NF-κB）p65 expression was detected by Western blotting while the expression of CX3CR1 was determined by immunohistochemical staining.Results：Compared with the sham group,the TWL and MWT of rats in the CCI group were significantly decreased on each post-operative day（P〈0.01）,which reached a nadir on the 3rd day after CCI,and the expressions of NF-κB p65and CX3CR1 were markedly increased in spinal cord dorsal horn and DRG.In the Cur group,the TWL of rats were significantly increased than those in the CCI group on post-operative day 7,10 and 14（P〈0.05）and MWT increased than those in the CCI group on post-operative day 10 and 14（P〈0.05）.In addition,the administration of curcumin significantly decreased the positive expressions of NF-κB p65 and CX3CR1 in spinal cord and DRG（P〈0.05）.Conclusion：Our study suggests that curcumin cou

关 键 词：curcumin neuropathic pain microglia nuclear factor κ B CX3C chemokine receptor 1 

分 类 号：R285.5[医药卫生—中药学]