老龄载脂蛋白E基因敲除小鼠血脂及病理组织学观察  

Observation of serum lipid and histopatholoical changes in elderly mice with apolipoprotein E knockout gene

作　　者：李凤卿[1] 孙婷婷[1] 王珺晓 罗善顺[1] 

机构地区：[1]哈尔滨医科大学附属第一医院心内科,黑龙江哈尔滨150001

出　　处：《心脏杂志》2014年第6期635-636,640,共3页Chinese Heart Journal

基　　金：国家自然科学基金项目资助(81070216);国家自然科学基金项目资助(81270366)

摘　　要：目的：观察用普食喂养而非以往研究中的高脂饮食喂养的载脂蛋白E基因敲除小鼠（ApoE-/-）的血脂及病理组织学变化特点。方法： 各选取20只8周龄雄性小鼠和同龄同性C57BL/6J小鼠为对照，普食喂养6-8个月，测定血清中总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）的含量。常规制备主动脉的病理切片，进行HE染色，观察病理学变化。结果： ApoE-/-小鼠血清中TC、TG、LDL含量明显高于对照组（P〈0.05），且ApoE-/-小鼠主动脉根部病理切片明显脂质斑块形成。结论： 普食喂养8月龄ApoE-/-小鼠是研究动脉粥样硬化（AS）的理想动物模型，为AS性心血管疾病研究者提供了参考资料。AIM：To observe serum lipid and histopathological changes in ApoE gene knockout mice fed a normal diet instead of a high-fat diet. METHODS： Twenty eight-week-old mice （male） with ApoE gene knockout were selected and 20 C57BL/6J mice （male） were used as controls. The mice in both groups were fed normal diets for 6-8 months and the levels of TC, TG and LDLwere measured in the two groups. Routine aortic pathological productions were stained by HE to observe the pathological changes. RESULTS： Levels of TC, TG and LDL-c in mice with ApoE knockout gene were significantly higher than those in control group （P〈0.05） and atherosclerotic plaques formed in ApoE gene knockout mice. CONCLUSION： ApoE gene knockout mice fed normal diets for 8 months are the ideal animal models for studies on atherosclerotic plaque research.

关 键 词：载脂蛋白E基因敲除小鼠 血脂 病理改变 

分 类 号：R543[医药卫生—心血管疾病]