Rapid Identification of Bacterial Species Associated with Bronchiectasis via Metagenomic Approach  

作　　者：YANG Dong Hong ZHANG Yuan Yuan DU Peng Cheng XU Li WANG Hai Yin HAN Na CHEN Chen GAO Zhan Cheng 

机构地区：[1]Department of Respiratory and Critical care medicine,Peking University People’s Hospital [2]State Key Laboratory for Infectious Disease Prevention and Control,and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention [3]Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

出　　处：《Biomedical and Environmental Sciences》2014年第11期898-901,共4页生物医学与环境科学（英文版）

基　　金：supported by grants from the Ministry of Science and Technology of China(National High-Tech R&D program:No.2006AA02Z4A9 and No.2012BAI05B02;12th Five-Year Plan Major Scientific and Technological Program:No.2012ZX10004-206);the Ministry of Health of China(Special Fund for Health-scientific Research in the Public Interest Program:No.201202011)

摘　　要：Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved.However,30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria,making it difficult to confirm the pathogen.In this study,we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation,and sent for 16 S rDNA pyrosequencing by a 454 GS Junior machine.Metagenomic analysis showed the composition of bacterial community in sample was complex.More than a half of reads（51.3%） were from Pseudomonas aeruginosa.This result was corresponding with the culture result but came out 2 d earlier,which is meaningful for early diagnosis and treatment.The detection with 16 S rDNA pyrosequencing technology is more sensitive and rapid than routine culture,and can detect the co-infection or symbiosis in airway,giving us a novel and convenient approach to perform rapid diagnosis.Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved.However,30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria,making it difficult to confirm the pathogen.In this study,we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation,and sent for 16 S rDNA pyrosequencing by a 454 GS Junior machine.Metagenomic analysis showed the composition of bacterial community in sample was complex.More than a half of reads（51.3%） were from Pseudomonas aeruginosa.This result was corresponding with the culture result but came out 2 d earlier,which is meaningful for early diagnosis and treatment.The detection with 16 S rDNA pyrosequencing technology is more sensitive and rapid than routine culture,and can detect the co-infection or symbiosis in airway,giving us a novel and convenient approach to perform rapid diagnosis.

关 键 词：pathogenic exacerbation meaningful earlier Junior giving airway routine Species Associated 

分 类 号：R562.22[医药卫生—呼吸系统]