番茄黄化曲叶病毒(TY)侵染性克隆接种鉴定方法研究  

Study on Inoculated Identification Techniques for Infectious Clones to Tomato Yellow Leaf Curl Virus (TY)

作　　者：张少丽[1] 邵景成[1] 胡志峰[1] 

机构地区：[1]甘肃省农业科学院蔬菜研究所,甘肃兰州730070

出　　处：《甘肃农业科技》2014年第10期16-19,共4页Gansu Agricultural Science and Technology

基　　金：甘肃省农业科学院农业科技创新专项(2012GAAS15-16)部分研究内容

摘　　要：采用YEP1和YEP2培养基培养番茄黄化曲叶病毒侵染性克隆pBinPLUS-1.7A＋2β,并用固体菌落、液体菌菌液浓度OD600≈0.6、液体菌菌液浓度OD600≈1.0对感病番茄品种MM、中蔬4号、98-B1在4~6叶苗期进行接种处理。结果显示,2种液体培养基的液体菌菌液浓度为OD600≈0.6时接种处理发病率较高,并初步确立了无TY发病地区侵染性克隆接种鉴定方法。The pBinPLUS-1.7A＋2β was nourished in two species medium,and set up three different treatments respectively,such as colonies,the two concentration of agrobacterium is about OD600≈0.6 or OD600≈1.0. The 4~6 leaves seedlings were inoculated in susceptible tomato varieties MM,Zhongshu 4,and 98-B1. The results shows that it was higher TY incidence when the two concentration of agrobacterium is about OD600≈0.6. The techniques of pBinPLUS-1.7A＋2β inoculation identification is established preliminarily in the areas,where the TY has not happened.

关 键 词：番茄 黄化曲叶病毒病 侵染性克隆 抗性鉴定 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]