30个InDel位点在华东汉族和畲族人群的法医学应用  

Forensic Application of 30 InDel Loci in Han and She Nationalities of Eastern China

作　　者：胡真[1,2] 王正[2] 张素华[2] 赵书民[2] 朱如心[2] 孙宽[2] 李成涛[2] 

机构地区：[1]苏州大学医学部法医学系,江苏苏州215123 [2]司法部司法鉴定科学技术研究所 上海市法医学重点实验室,上海200063

出　　处：《法医学杂志》2014年第5期337-341,345,共6页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81302026;81222041);"十二五"国家科技支撑计划项目(2012BAK16B01)

摘　　要：目的评估InvestigatorDIPplex试剂盒中30个插入/缺失(insertion/deletion,InDel)多态性位点在华东汉族和畲族人群的法医学应用价值。方法采用InvestigatorDIPplex试剂盒对华东地区565名汉族和119名畲族无关个体进行分型检测,统计分析30个位点的等位基因频率和群体遗传学参数。结果在华东汉族人群中平均Ho为0.413 3,平均DP为0.551 1,平均PIC为0.320 0。在畲族人群中平均Ho为0.389 6,平均DP为0.543 3,平均PIC为0.310 0。30个位点在汉族、畲族人群中均符合Hardy-Weinberg平衡(P>0.05)。结论 InvestigatorDIPplex试剂盒包含的30个位点在华东汉族和畲族中多态性良好,在特殊亲权鉴定案件中可作为良好的补充体系。Objective To evaluate the forensic application value of 30 insertion/deletion （InDel） loci in- cluded in Investigator（R） DIPplex Kit in Han and She nationalities of Eastern China. Methods A total of 565 unrelated individuals in Han nationality and 119 ones in She nationality of Eastern China were in- vestigated using Investigator（R） DIPplex Kit. Allele frequencies, population genetics parameters of the 30 InDel loci were statistically calculated. Results In Han nationality, the mean Ho was 0.413 3, the mean DP was 0.551 1, the mean PIC was 0.320 0. And in She nationality, the mean Ho was 0.389 6, the mean DP was 0.543 3, the mean PIC was 0.3100. No deviation from Hardy-Weinberg equilibrium was observed in Han and She nationalities （P〉0.05）. Conclusion The 30 loci in Investigator（R） DIPplex Kit show good genetic diversity in Han and She nationalities, and could be used as a supplemental tool for some special paternity cases.

关 键 词：法医遗传学 多态现象 遗传 插入/缺失 Investigator(R) DIPplex试剂盒 汉族 畲族 

分 类 号：DF795.2[医药卫生—法医学]