异养小球藻半连续发酵生产油脂工艺探讨  

Explore the Oil Production Process of Chlorella Vulgaris in Semi Continuous Fermentation

作　　者：毕生雷 张成明[2,3] 李十中[2,3] 刘钺[1] 杜风光[1] 

机构地区：[1]河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,河南南阳473000 [2]清华大学核能与新能源技术研究院,北京100084 [3]北京市生物燃料工程技术研究中心,北京100084

出　　处：《食品与发酵科技》2014年第5期36-40,48,共6页Food and Fermentation Science & Technology

基　　金："十二五"农村领域国家科技支撑计划课题(2011BAD14B05);国家科技支撑计划(2012BAC18B01);国家国际科技合作项目(2010DFB64040)的资助

摘　　要：研究了异养小球藻半连续发酵工艺,并对各个生长阶段的细胞数变化、干重变化、单位时间干重变化做了对比。结论:采用半连续发酵工艺培养异养小球藻时,平均干重增加速率为8.395g/(L·d),与传统发酵工艺相比,提高22.38%;平均发酵周期仅216h,与传统发酵工艺相比,发酵周期缩短30.32%;发酵110h后,藻细胞干重质量浓度均能达到100g/L以上,且油脂含量与传统发酵工艺相差不大。采用半连续发酵工艺,可以提高异养小球藻的整体发酵效率,有利于规模化应用。The semi-continuous fermentation process of the heterotrophic Chlorella was studied, and the changes of cell number, dry weight production, and dry weight productivity for each growth stage were investigated. Results： using the semi-continuous fermentation process to cultivate the heterotrophic Chlorella, the average daily dry weight production and fermentation time was 8.395g/（Lod） and 216 h, which was 22.38% higher and 30.32% shorter than that of the traditional fermentation process, respectively. In semi-continuous fermentation process, the dry weight could achieve more than 100g/L after 110h, and the oil content was similar to that obtained by the traditional fermentation process. Semi-continuous fermentation could improve the fermentation efficiency of heterotrophic Chlorella, and is more conducive to the application on industrial scale.

关 键 词：小球藻 半连续发酵 

分 类 号：TQ645[化学工程—精细化工]