金钗石斛种子萌发率提高及可诱导愈伤组织培养基的研究  

Germination rate promotion and callus induction via tissue culture technique in Dendrobium nobile Lindle

作　　者：徐德林[1] 蔡振锋[1] 张林[1] 储士润 祝飞[2] 钱刚[1] 

机构地区：[1]遵义医学院细胞生物学与遗传学教研室,贵州遵义563099 [2]遵义医学院第一临床学院,贵州遵义563099

出　　处：《遵义医学院学报》2014年第5期541-545,共5页Journal of Zunyi Medical University

基　　金：贵州省中药现代化科技产业研究开发专项(NO:黔科合ZY字[2013]3002);贵州省优秀科技教育人才省长基金项目(NO:黔省专合字2008-61);遵义医学院2011年招标项目(NO:F-551);遵义医学院2012年招标项目(NO:F-611)

摘　　要：目的提高金钗石斛种子的萌发率,探索诱导愈伤组织的培养基。方法以金钗石斛种子为材料,研究其种子的萌发和愈伤组织的诱导条件。结果金钗石斛种子在MS+土豆块10 g/L+琼脂7 g/L+蔗糖30 g/L的培养基中萌发最快,时间为9 d,萌发率最高达92.5%;培养基(MS+0.2 g/L碳颗粒+琼脂7 g/L+蔗糖30 g/L)能够诱导金钗石斛原球茎形成愈伤组织。结论在MS培养基中单独添加土豆时能提高金钗石斛成熟种子的萌发率;碳粉能有效促进金钗石斛愈伤组织的形成。Objective To promote the germination rate and to obtain the callus of Dendrobium nobile Lindle, we explored the media components through tissue culturing. Methods The seeds of D. nobile were applied to observe the germination condition and the callus induction in tissue culture. Results High germination rate （92.50%） was screened from the culture medium with the shortest time of 9 days, i.e. Murashige and Skoog medium （MS） ＋ 10 g/L potato pieces ＋ 7 g/L agar ＋ 30 g/L sucrose. Moreover, the callus was induced from the optimized culture medium as MS ＋ 0.2 g/L carbon powder ＋ agar 7 g/L ＋ 30 g/L sucrose. Conclusion The germination rate could he significantly promoted in MS medium combination with potato. And carbon powder probably improves the callus induction in D. nobile.

关 键 词：金钗石斛 组织培养 种子萌发率 愈伤组织 

分 类 号：Q949.95[生物学—植物学]