ICU鲍氏不动杆菌耐药性分析及同源性调查  

Drug resistance of Acinetobacter baumannii strains isolated from ICUs and analysis of homology

作　　者：陈佑明[1] 孙恒彪[1] 张婧[1] 黄敬[1] 杜凌燕[1] 黎玉伦[2] 

机构地区：[1]南方医科大学第三附属医院检验科,广东广州510630 [2]南方医科大学第三附属医院医院感染管理科,广东广州510630

出　　处：《中华医院感染学杂志》2014年第22期5477-5479,共3页Chinese Journal of Nosocomiology

基　　金：广州市科技计划基金资助项目(2012Y5-00004)

摘　　要：目的统计分析重症监护病房(ICU)不同患者送检标本及环境中分离得到的鲍氏不动杆菌耐药性及同源性,为临床治疗及医院感染管理科感染控制提供依据,切断鲍氏不动杆菌医院感染传播途径。方法应用西门子MicroScan微生物鉴定及药敏系统对来自ICU患者及环境中的鲍氏不动杆菌进行细菌培养鉴定及药敏分析,运用肠杆菌科基因组间重复序列聚合酶链反应(ERIC-PCR)及琼脂糖凝胶电泳进行同源性分析。结果 13株鲍氏不动杆菌中有10株为多药耐药株,其中7株对常用抗菌药物均耐药,有3株分离自ICU患者送检标本、4株分离自ICU环境;同源性分析显示,13株鲍氏不动杆菌共分为4型(A^D),其中有10株为A型。结论 ICU鲍氏不动杆菌聚集暴发由A1亚型引起,含有鲍氏不动杆菌的气溶胶可能为主要传播途径。OBJECTIVE To statistically analyze the drug resistance of the Acinetobacter baumannii strains isolated from the submitted specimens that were obtained from patients in the ICUs or the environment and observe the ho‐mology so as to provide guidance for clinical treatment and control of nosocomial infections and cut the transmis‐sion route of the A .baumanni infection .METHODS The bacterial culture and identification were performed for the A .baumanni strains that were isolated from the ICU patients and environment by using Siemens MicroScan micro‐bial identification system ,the drug susceptibility was analyzed with the use of drug susceptibility system ;the ho‐mological analysis was performed by means of the Enterobacterial Repetitive Intergenic Consensus‐Polymerase Chain Reaction （ERIC‐PCR） and agarose gel electrophoresis .RESULTS Of 13 strains of A .baumannii ,10 strains were multidrug‐resistant ,of which 7 strains were resistant to the commonly used antibiotics ,including 3 strains isolated from the submitted specimens of the ICU patients and 4 strains isolated from the environment of ICU .The homological analysis indicated that the 13 strains of A .baumannii were divided into four types （from type A to D） ,and there were 10 strains of type A .CONCLUSION The cluster outbreak of the A .baumanni strains in the ICUs is induced by the subtype A1 ,and the aerosols harboring in the A .baumanni may be the major transmission route .

关 键 词：鲍氏不动杆菌 重症监护病房 医院感染 耐药性 同源性 

分 类 号：R378[医药卫生—病原生物学]