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作 者:KOH Hung Sun KWEON Gu Hee HAM Eui Jeong JANG Kyung Hee YANG Beong Guk AN Na Hyeon
机构地区:[1]Department of Biology, Chungbuk University, Cheongju 361-763, Korea [2]National Institute of Environmental Research, Incheon 404 - 708, Korea
出 处:《兽类学报》2014年第4期374-380,共7页Acta Theriologica Sinica
摘 要:利用韩国豹猫的IRBP、12S rRNA和细胞色素b(Cyt b)序列,并将其与Gen Bank中的豹猫及西表猫的上述基因进行比较,以此来重新分析韩国豹猫和其它豹猫及西表猫之间的遗传分化情况。首先,我们从18只韩国豹猫的IRBP、12S rRNA和Cyt b序列分析中分别检测到6个等位基因、1个单倍型和3个单倍型,推测曾经发生在豹猫种群的瓶颈事件可能是本文发现的不同分子标记之间存在基因型丢失差异的原因。基于Cyt b序列分析,证实东亚的豹猫形成一个单独的进化枝,本文得到的Cyt b序列和之前发布结果支持一个分类理论,即认为东亚的豹猫就是东北森林猫。我们发现在IRBP、12S rRNA基因上,韩国豹猫和西表猫存在相同的序列,支持将西表猫和豹猫视为同一物种。We obtained IRBP,12 S rRNA,and Cytochrome b( Cyt b) sequences of the leopard cat( Prionailurus bengalensis) from Korea,and these sequences were compared to the corresponding sequences of the leopard cat and the Iriomote cat,P. iriomotensis,obtained from Gen Bank,to reexamine genetic divergence of Korean leopard cats from both other leopard cats and Iriomote cats. First,from IRBP,12 S rRNA,and Cyt b sequencing analyses with 18 Korean leopard cats,six alleles,one haplotype,and three haplotypes were identified,respectively,and we concluded that past bottleneck events that occurred in Korean leopard cats,is a plausible cause for the differential loss of genotypes detected in this study. Based on Cyt b sequence analysis we confirmed that East Asian leopard cats form a clade,and we found that former and present Cyt b sequencing results support a previous classification that recognized P. bengalensis from East Asia as P. euptilurus. Finally,we detected identical sequences in the IRBP and 12 S rRNA genes between specimens of P. bengalensis from Korea and those of P. iriomotensis,supporting widespread treatment of the Iriomote cat as conspecific with P. bengalensis.
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