不同剂量瑞舒伐他汀对氧化修饰低密度脂蛋白诱导单核-巨噬细胞的肝X受体及小凹蛋白1表达的影响  

Effects and mechanism of different doses of rosuvastatin on expression of liver X receptor and caveolin-1 in cultured human monocyte-macrophage cells induced by oxidized low density lipoprotein

作　　者：卫政[1] 武永新[1] 柳丽娜[1] 吉鹏娟 申晓彧[1] 

机构地区：[1]山西医科大学第二医院心内科,太原030000

出　　处：《中国综合临床》2014年第11期1138-1141,共4页Clinical Medicine of China

基　　金：山西省科技攻关项目（20120313020-10）

摘　　要：目的 研究不同剂量瑞舒伐他汀对氧化修饰低密度脂蛋白（ox-LDL）诱导的人单核巨噬细胞肝X受体及小凹蛋白1表达的影响.方法 实验分6组：空白对照组、ox-LDL对照组、不同剂量（0.01μmol/L,0.1μmol/L,1.0μmol/L,5.0 μmol/L）瑞舒伐他汀组,RT-PCR法测定各组肝X受体及小凹蛋白1mRNA的表达.结果 空白对照组与ox-LDL对照组肝X受体mRNA的表达分别为1.00±0.02与0.26±0.02,两组比较差异有统计学意义（t=56.392,P＜0.001）;小凹蛋白1mRNA分别为1.00±0.04与0.27±0.01,两组比较差异有统计学意义（t=31.278,P＜0.001）.ox-LDL对照组及不同剂量瑞舒伐他汀组间肝X受体及小凹蛋白1mRNA表达比较,差异均有统计学意义（F值分别为72.154、66.007,P均＜0.001）,随瑞舒伐他汀剂量的增加,肝X受体及小凹蛋白1mRNA表达呈上升趋势,两两比较差异均有统计学意义（P均＜0.05）.结论 瑞舒伐他汀可上调单核-巨噬细胞肝X受体mRNA、小凹蛋白1mRNA的表达,且呈剂量依赖性.Objective To investigate the effects of different doses of rosuvastatin on expression of liver X receptor（LXR） and caveolin-1 in cultured human monocyte-macrophage cells which induced by oxidized low density lipoprotein （ox-LDL）.Methods The human monocyte-macrophage cells were divided into six groups：control group,ox-LDL group,different doses of rosuvastatin group （0.01 μmol/L,0.1 μmol/L,1 μmol/L,5 μmol/L）.The expression of LXR mRNA and caveolin-1 mRNA were assayed by RT-PCR.Results LXR mRNA expression induced by ox-LDL in the control group and ox-LDL group were 1.00 ± 0.02,0.26 ± 0.02,and the difference was significant （t =56.39,P ＜ 0.001）.Meanwhile,caveolin-1 mRNA expression in ox-LDL is （0.27 ± 0.01） fold than that in control （t =31.27,P ＜ 0.001）.Meanwhile,There were significant differences among ox-LDL group and the different doses of rosuvastatin group in terms of LXR mRNA and caveolin-1 mRNA expressions （F =72.154,66.007,P ＜ 0.001）.Along with the increase the doses of rosuvastatin,there was an increased trends of LXR mRNA and caveolin-1 mRNA expressions （P ＜ 0.05）.Conclusion Rosuvastatin and upregulated the LXR mRNA and caveolin-1 mRNA expressions in a dose dependent manner.

关 键 词：瑞舒伐他汀 氧化修饰低密度脂蛋白 肝X受体 小凹蛋白1 胆固醇逆转运 

分 类 号：R96[医药卫生—药理学]