不同温度下白僵菌Bb04菌株对红火蚁工蚁的致病力  

Pathogenicity of strain Bb04 of Beauveria bassiana( Bals) Vuill.to red imported fire ant workers under different temperatures

作　　者：刘晓燕[1] 吕利华[2] 何余容[1] 

机构地区：[1]华南农业大学红火蚁研究中心,广东广州510642 [2]广东省农业科学院植物保护研究所,广东广州510640

出　　处：《生物安全学报》2014年第2期112-116,共5页Journal of biosafety

基　　金：广东省科技计划项目(2007B020710014);广东省自然科学基金(06025386)

摘　　要：[背景[白僵菌是一种应用最广泛的虫生真菌,已被用于工厂化大量生产,可防治多种农林害虫。[方法]试验设置17、21、25、29和33℃5个温度水平,采用喷雾法,将浓度为1×105和1×108个·mL-1的白僵菌孢子悬浮液分别感染红火蚁工蚁,以测试不同温度下白僵菌菌株对红火蚁工蚁的致病力。[结果]当浓度为1×108个·mL-1时,在21、25和29℃条件下,红火蚁工蚁在15 d的累计死亡率均达到了100%,在17和33℃下分别为99.36%和98.74%。当浓度为1×105个·mL-1时,在21、25、29和33℃下,红火蚁工蚁在15 d的累计死亡率分别为29.42%、36.18%、33.17%和27.21%,显著高于17℃的累计死亡率。2个浓度处理在17～25℃时,白僵菌对红火蚁工蚁的致死中时（LT50）随着温度的升高而缩短,当温度为29和33℃时,红火蚁的LT50不减少反而增加。25℃时白僵菌Bb04菌株对红火蚁工蚁的LT50最短,死亡速度最快,致病力最强。[结论与意义]该研究可为制定田间利用白僵菌防治红火蚁的最佳时期奠定基础。[ Background ] Red imported fire ant （RIFA）, Solenopsis invicta Buren, is one of the most devastating pests in the world. Entomopathengenic fungi have been applied to control many crops insect pests successfully worldwide and may be a promising control for RIFA. The effect of temperature on pathogenicity of strain Bb04 of Bearveria bassiana, a suitable candidate to the RIFA workers, was studied in the laboratory. [ Method] The RIFA workers were infected with two concentrations of B. bassiana spore suspension （1 × l05 conidia · mL-1 and 1 × 105 conidia · mL-1） and kept under 17, 21, 25, 29 and 33 ℃ conditions. [ Result]The results showed there were significant differences in virulence of Bb04 to RIFA under different temperatures. When workers of RIFA were treated with 1 × 10s conidia · mL-1 , the total mortality of infected RIFA after 15 days reached as high as 100% under 21 ℃, 25 ℃ and 29 ℃. Total mortality was 99.36% at 17 ℃ and 98.74% at 33 ℃. However, after 15 days with an infection rate of 1 × 105 conidia/mL, total mortalities of treated workers at 17 ℃, 21 ℃, 25 ℃, 29 ℃ and 33 ℃ were only 16.83% , 29.42% ,36.18%, 33.17%and 27.21%, respectively. At 17, 21 and 25 ℃, LTso values of Bb04 against RIFA decreased from 5.41 at 17 ℃ to 3.88 at 21 ℃ and then 2.57 days at 25 ℃. LT50 increased again to 2.97 at 29 ℃ and 5.11 at 33 ℃. [Conclusion and significance]25 ℃ was the most effective temperature to promote infection of RIFA by Bb04 and should be applied in both spring and autumn in the field.

关 键 词：白僵菌Bb04菌株 红火蚁 温度 致病力 

分 类 号：S476[农业科学—农业昆虫与害虫防治]