藏药红花绿绒蒿中木犀草素的定性定量分析  

Identification and Determination of Luteolin in Meconopsis punicea Maxim

作　　者：余成龙[1] 宋良科[1] 吴蜀星[1] 曹雨虹[1] 李治昊[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《现代中药研究与实践》2014年第5期26-29,共4页Research and Practice on Chinese Medicines

基　　金：国家中医药管理局行业科研专项(201107009)

摘　　要：目的以红花绿绒蒿Meconopsis punicea Maxim.黄酮类化学组分的定性定量分析为基础,确定药材的主要指标性化学成分,为药材质量标准的制定奠定基础。方法采用薄层色谱法确定红花绿绒蒿中的主要化学组分;采用高效液相色谱外标法进行指标性化学成分的含量测定,以Chrompsuper C18(4.6mm×250mm,5μm)为色谱柱,甲醇-0.2%磷酸溶液(60:40)为流动相,流速为0.6ml·min-1,检测波长为349nm,柱温为30℃。结果薄层色谱法可检出木犀草素,木犀草素的回归方程为Y=183250X+48493(R2=0.9995),木犀草素在0.084μg^0.84μg范围内呈良好的线性关系,平均加样回收率为97.82%,RSD为1.01%。结论该定性定量分析方法稳定、重复性良好,可作为红花绿绒蒿药材质量的评价方法之一,为进一步制定药材质量标准提供了依据。Objective The major indicative chemical constituent of Meconopsis punicea Maxim. was confirmed based on the identification and determination of luteolin in Meconopsis punicea Maxim. Methods The major chemical constituents were confirmed by the TLC method, the indicative chemical constituent was determined by the HPLC method. The HPLC was confirmed using Chrompsuper Cls column （4.6mm×250mm, 5μm） and methanol -0.2% orthophosphoric acid （60：40）as the mobile phase, the flow rate was 0.6ml·min-1, the detection wavelength was 349nm, and the column temperature was 30 ℃. Results The luteolin was identified by the TLC method, the regression equation of luteolin was Y=183 250X＋48 493 （R2=0.999 5）, the linear range of luteolin was 0.084kμg - 0.84μg, the average recovery was 97.82% with the RSD was 1.01%. Conclusion The method is stable and iterative, the method can be used to evaluate the quality of Meconopsis punicea Maxim., the method provides foundation for further research on the quality standard of Meconopsis punicea Maxim..

关 键 词：红花绿绒蒿 薄层色谱法 高效液相色谱法 木犀草素 

分 类 号：R284.1[医药卫生—中药学]