基于UV法优化铁棒锤种子生物碱提取工艺测定总碱含量  

Study on Extraction Technology of Aconitum flavum Seed Alkaloids and Content Determination of Total Alkaloids Based on UV

作　　者：林丽[1,2] 杨建成 高科艳 王耀鹏[5] 

机构地区：[1]甘肃中医学院教学实验中心,甘肃兰州730000 [2]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000 [3]甘肃省医药学校,甘肃兰州730020 [4]兰州太宝制药有限公司,甘肃兰州730050 [5]甘肃中医学院药学院,甘肃兰州730000

出　　处：《现代中药研究与实践》2014年第5期55-57,共3页Research and Practice on Chinese Medicines

基　　金：甘肃教育厅科研项目(0906B 07);甘肃中医学院中青年科研基金项目(09ZQ 17);(1106B-02);2012年大学生创新项目

摘　　要：目的建立铁棒锤种子中生物碱的最佳提取方法,为铁棒锤种子生物碱的有效利用提供科学依据。方法采用生物碱显色反应、薄层色谱法对铁棒锤种子生物碱类成分进行定性鉴别;采用正交实验设计,运用紫外分光光度法进行定量分析。结果总生物碱的最佳提取工艺条件:提取时间30 min,乙醇浓度60%,料液比1:20。铁棒锤种子总生物碱含量为0.19%。结论方法简便,超声提取法提取率高,有可利用价值,可为铁棒锤种子化学成分进一步研究提供科学依据。Objective To establish the best extraction method for the alkaloids in Aconitum flavum seeds,and provide scientific basis for the effective use of Aconitum flavum seed alkaloid.Methods By the chromogenic reaction of the alkaloid and TLC for the qualitative identification of Aconitum flavum seed alkaloids;the orthogonal experimental design and UV spectrophotometry were used for quantitative analysis.Results The optimal extraction method was as follows：extraction 30rain,concentration of ethanol 60%,ratio for solid-liquid 1：20. The content of total alkaloids was 0.19%.Conclusion The method is simple with high extraction rate of ultrasonic extraction and has utility value,which can provide scientific basis for further studies on chemical constituents of Aconitum flavum seeds.

关 键 词：UV 铁棒锤种子 提取工艺 总生物碱 

分 类 号：R284.2[医药卫生—中药学]