黔产续断HPLC指纹图谱研究  

HPLC Fingerprint Analysis of Dipsacus asperoides Produced from Guizhou

作　　者：魏升华[1] 田红红[2] 杨卫灵 周岚[2] 伍庆[2] 李朝婵[2] 

机构地区：[1]贵阳中医学院,贵州贵阳550001 [2]贵州师范大学,贵州贵阳550001

出　　处：《贵州农业科学》2014年第10期45-47,50,共4页Guizhou Agricultural Sciences

基　　金：国家科技支撑项目"巫山淫羊藿质量标准提升及其综合利用研究"(2011BAI13B04-01);"半夏;续断;金钗石斛等八种药材规范化种植和野生保护抚育关键技术研究及应用示范"(2009BAI74B01-031);贵阳市科技计划项目(2012工业振兴科技计划)"术科考试物联网智能应用系统"[筑科合同(2012101)12]

摘　　要：为建立贵州续断药材的指纹图谱,采用D101大孔树脂对样品进行前处理,用Agilent ZORBAX extend C18(250mm×4.6mm,5μm)色谱柱进行梯度洗脱,采用SPSS19.0进行数据分析,对贵州不同产地的续断药材进行指纹图谱构建。结果表明:11批续断样品有14个共有峰,方法学考察符合指纹图谱技术要求;聚类分析聚为2类,贵州不同产地续断药材指纹图谱相似度极高,建立的续断指纹图谱测定方法准确可靠。In order to establish the chromatographic fingerprint of Dipsacus asperoides produced from Guizhou,samples were pre-treated by D101macroporous resin,agilent ZORBAX extend C18 （250 mm 4.6 mm,5 μm）column was used for gradient elution,and SPSS 19.0 was used for data analysis.The results showed that the 11 batches of D.asperoides had 14 common peaks,the methodological study results met the requirements of chromatographic fingerprint.According to system clustering analysis,it could be divided into 2 categories. The similarity of chromatographic fingerprint among D.asperoides from different origins in Guizhou was extremely high.Therefore,the determination method in the study could be approved accurate and reliable.

关 键 词：续断 HPLC 指纹图谱 质量控制 贵州 

分 类 号：S567[农业科学—中草药栽培] R284.2[农业科学—作物学]