火龙果黑腐病病原鉴定与ITS序列分析  

Identification and ITS Sequence Analysis of Black Rot Pathogen of Pitaya

作　　者：郑伟[1] 王彬[1] 彭丽娟[2] 李兴忠[1] 

机构地区：[1]贵州省果树科学研究所,贵州贵阳550006 [2]贵州大学,贵州贵阳550025

出　　处：《贵州农业科学》2014年第10期115-117,共3页Guizhou Agricultural Sciences

基　　金：贵州省重大科技专项"火龙果产业化关键技术研究与集成示范"子课题-5[黔科合重大专项字(2012)6006];贵州省农业科技成果转化资金项目"火龙果新品种紫红龙转化与示范"(2012GB2F20040);贵州省科技创新人才团队建设项目"贵州省精品火龙果研究科技创新团队"[黔科合人才团队(2013)4005];贵州省农科院专项"火龙果病虫害调查及防治技术研究"[黔农科院院专项(2008)013]

摘　　要：为了对火龙果黑腐病的防治提供理论依据,对从火龙果黑腐病病株上分离得到的病原菌进行形态特征、致病性以及核糖体DNA.ITS序列分析。结果表明,引起火龙果黑腐病的病原只有1种,其分生孢子单生,梭形,PDA培养基上菌落生长圆形;克隆分析菌株的核糖体DNA.ITS区域序列,该病原菌与B.cactivora的菌株同源性高达100%。引起火龙果黑腐病病原菌为半知菌亚门丝孢纲丝孢目平脐蠕孢属(Bipolaris)的B.cactivora。The morphological characteristics,pathogenicity and sequence of ribosoml DNA-ITS of the pathogenic bacteria isolated from disease pitaya plants with black rot were identified and analyzed to provide the theoretical basis for control of black rot in pitaya.The results showed that the pathogen to cause black rot of pitaya is only one,its conidium is single and fusiformis,and its colony on PDA medium is roundness.The homology between the pathogen and B.cactivora strain is up to 100% by analyzing the sequence of ribosoml DNA-ITS.The pathogen to cause black rot of pitaya is identified as B.cactivora, Bipolaris,Moniliales,Hyphomyceles,Deuteromycotina according to its morphological characteristics.

关 键 词：火龙果 黑腐病 rDNA-ITS序列 病原鉴定 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]