盐胁迫下褐环粘盖牛肝菌(sp9)质膜ATPase的活性  

Effect of Different NaCl Stress on ATPase Activity of Plasma Membrane of Suillus luteus(sp9)

作　　者：李春玲[1] 陈有君[1] 李国光[1] 孙琳[1] 黄蕾[1] 

机构地区：[1]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018

出　　处：《贵州农业科学》2014年第10期167-170,共4页Guizhou Agricultural Sciences

摘　　要：为探明褐环粘盖牛肝菌调节高盐的培养机理,通过设置不同pH培养条件,研究了盐胁迫对褐环粘盖牛肝菌（sp9）质膜ATPase活性的影响。结果表明：在初始pH4~6.5条件下,添加100 mmol/L NaCl组菌丝细胞质膜的H＋-ATPase、K＋-ATPase和Ca2＋-ATPase活性都较对照组有一定程度的升高,培养基pH比对照组下降幅度大,且在初始pH5.5~6.5时其活性变化更明显。一定浓度的NaCl,会对不同pH培养的褐环粘盖牛肝菌生长代谢产生影响,且会通过H＋-ATPase、K＋-ATPase、Ca2＋-ATPase、载体和各种离子通道蛋白协调启动自身调节机制,以缓解外界对其造成伤害。The ATPase activity of plasma membrane of Suillus luteus was determined under different NaCl stress to study the effect of different NaCl stress on ATPase activity of plasma membrane of Suillus luteus.The results showed that H＋-ATPase,K＋-ATPase and Ca2＋-ATPase activity of mycelium plasma membrane of Suillus luteus cultured on the medium with adding 100mmol/L NaCl is higher than CK under initial pH 4 ~ 6.5 to some degree,and pH of the medium with adding NaCl significantly decreases compared with CK,especially change of the ATPase activity is obvious under initial pH 5.5~6.5.Certain NaCl concentration has the effect on growth and metabolism of Suillus luteus cultured on media with different pH but the damage from the environment can be alleviated by the autogenous regulation mechanism started by coordination of H＋-ATPase,K＋-ATPase,Ca2＋-ATPase,vectors and different ion channel protein.

关 键 词：褐环粘盖牛肝菌 ATPASE活性 NACL 盐胁迫 

分 类 号：S646.3[农业科学—蔬菜学]