仿刺参3个地理群体ITS1序列变异及系统发生分析  

Variation in ITS1 sequences and phylogenetic analysis of three geographical stocks of sea cucumber Apostichopus japonicus

作　　者：姬南京[1] 常亚青[1] 高银雪 赵冲[1] 宋坚[1] 

机构地区：[1]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2014年第5期449-453,共5页Journal of Dalian Ocean University

基　　金：国家自然科学基金资助项目(31072230);国家"863"计划项目(2012AA10A412)

摘　　要：利用核糖体DNA第一内转录间隔区（Internal transcribed spacer 1,ITS1）序列,对中国大连（CD）、朝鲜罗津（KN）和俄罗斯海参崴（RV）仿刺参Apostichopus japonicus 3个群体的遗传结构进行分析。结果表明：经PCR扩增、克隆测序,获得长度为517~519 bp、524 bp两种类型的ITS1核苷酸序列,平均G＋C含量（64.5%）显著高于A＋T含量（35.5%）;在仿刺参30个个体61条序列中共检测到49个变异位点,多态位点比例为9.33%,其中有4个简约信息位点,共有40种基因型,群体共享基因型为2个;遗传多样性分析表明,3个群体的遗传多样性丰富;分子方差分析显示,88.65%的变异来自于群体内部,群体间遗传分化较弱或中度分化;根据群体间遗传距离进行的聚类分析发现,KN群体与RV群体的亲缘关系较近,CD群体与KN、RV群体的亲缘关系较远。The genetic diversity and genetic differentiation were studied in three wild populations of sea cucumber Apostichopus japonicus from Dalian China （ CD） ,Rajin North Korea （ KN） and Vladivostok Russia（ RV） by internal transcribed spacer 1（ITS1）. Two different length of ITS1 sequence （517-519 bp and 524 bp） were obtained via cloning and sequencing of PCR products, with higher mean contents of G＋C （ 64. 5%） than those of A＋T （35. 5%）. There were 49 variation sites from 61 sequences in the 3 populations, with polymorphic sites of 9. 33%, and 4 parisimony informative among 40 genotypes including 2 shared genotypes. Genetic diversity parame-ters showed high genetic diversity among populations. The fixation indices （FST） of analysis of molecular variance （AMOVA） within populations was found to be 88. 65%,indicating that the three populations had weak or only moderately genetic differentiation. The molecular phylogenetic tree constructed with NJ method based on genetic distance revealed that there was closer linkage between KN population and RV population than between them and CD population.

关 键 词：仿刺参 ITS1序列 遗传多样性 遗传分化 

分 类 号：Q347[生物学—遗传学] S917.4[农业科学—水产科学]