化学发光免疫分析与酶联免疫分析法检测水产品药物残留的比较研究  

Detection of drug residues in fish and fishery products: comparison between chemiluminescence enzyme-linked immunoassay and enzyme linked immunosorbent assay

作　　者：萨仁托雅[1] 张峰[1] 郑有虎 卢亚楠[1] 

机构地区：[1]大连海洋大学水产与生命学院辽宁省海洋生物资源恢复与生境修复重点实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2014年第5期486-491,共6页Journal of Dalian Ocean University

基　　金：辽宁省农业攻关计划项目(2011215004);国家自然科学基金资助项目(30471323)

摘　　要：对检测水产品中呋喃唑酮代谢物（AOZ）和氯霉素（CAP）药物残留的两种方法——化学发光免疫分析（CLEIA）和酶联免疫分析（ELISA）法进行了比较研究。结果表明：两种药物残留检测中,CLEIA分析方法的线性范围和检出限均优于ELISA法,且两种免疫分析法的添加回收率均具有很好的相关性;用CLEIA法检测AOZ的检出限为0.01μg/kg,线性范围为0.01~2.56μg/kg,检测CAP的检出限为0.016μg/kg,线性范围为0.025~6.400μg/kg;用ELISA法检测AOZ的检出限为0.1μg/kg,线性范围为0.10~1.62μg/kg,检测CAP的检出限为0.05μg/kg,线性范围为0.05~4.05μg/kg;用CLEIA法检测CAP的批内变异系数（RSD）为5.5%~11.3%,批间RSD为12.3%~20.9%,检测AOZ的批内RSD为6.6%~11.1%,批间RSD为15.6%~18.3%。研究表明,用CLEIA法检测水产品药物残留较ELISA法检出限低、线性范围宽,虽然检出CAP和AOZ的变异系数均高于ELISA法,但仍符合《兽药残留酶联免疫试剂（盒）备案参考评判标准》的规定。Detection of furazolidone metabolite （ AOZ ） and chloramphenicol （ CAP ） residues in fish and fishery products was comparatively studied by chemiluminescence enzyme-linked immunoassay （ CLEIA ） and traditional colorimetric enzyme-linked immunosorbent assay （ ELISA） . The results showed that CLEIA had better linear range and detection limit than ELISA, and a close correlation of recovery rate was shown in the two methods. In CLISA, the detection limit was about 0. 01 μg/kg for AOZ, and about 0. 016 μg/kg for CAP and the linear range was from 0. 01 to 2. 56μg/kg for AOZ, and from 0. 025μg/kg to 6. 400μg/kg for CAP. In ELISA, however, the detection limit was 0 . 1 μg/kg for AOZ and about 0 . 05 μg/kg for CAP and the linear range was from 0 . 1 to 1 . 62 μg/kg for AOZ and from 0. 05 to 4. 05 μg/kg for CAP. The satisfactory precisions of the assay were found in CLEIA, with variation coefficient of 5. 5%-11. 3% in the intra-assay and 12. 3%-20. 9% in the inter-assay for CAP, and with variation coefficient of 6. 6-11. 1% in the intra-assay and 15. 6%-18. 3% in the inter-assay for AOZ, within the record reference evaluation standard of ELISA residues of veterinary drugs （ kit） .

关 键 词：化学发光免疫分析 酶联免疫分析 氯霉素 呋喃唑酮代谢物 

分 类 号：Q954.4[生物学—动物学]