高速逆流色谱法分离珠子草中的木脂素  

Isolation of lignans from Phyllanthus niruri Linn by high-speed countercurrent chromatography

作　　者：陈剑[1] 莫银屏 胡吉卓 刘志平[1] 韦万兴[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《广西大学学报（自然科学版）》2014年第5期1191-1197,共7页Journal of Guangxi University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(81060261);广西高校大学生创新创业计划项目(1301053);广西自然科学基金资助项目(2012GXNSFAA053021)

摘　　要：为克服采用传统方法分离木脂素的不足,采用高速逆流色谱法对珠子草中的木脂素进行分离,得到3个木脂素,通过红外光谱(IR)、质谱(MS)、核磁共振(NMR)等方法鉴定为珠子草素(niranthin)、叶下珠次素(hypophyllanthin)和叶下珠脂素(phyllanthin)。分离溶剂体系为正己烷/乙酸乙酯/甲醇/水(3/2/3/2,V/V),下相为流动相,流速为2 mL/min,转速为正转800 r/min,温度为25℃。实验结果为木脂素的分离提供了一条有效的途径。To overcome disadvantages of traditional lignans isolation methods,high-speed countercur-rent chromatography （ HSCCC） was employed and three lignans were isolated from Phyllanthus niru-ri Linn,which were identified as niranthin,hypophyllanthin,and phyllanthin by spectroscopic meth-ods,including infrared spectroscopy（IR）,mass spectrometry（MS）and nuclear magnetic resonance （ NMR） .Separating processes were given as following：light phase as stationary phase and heavy phase as mobile phase from mixture of n-hexane,ethyl acetate,methanol and water（3/2/3/2,V/V） with flow rate 2 mL/min,rotation speed 800 r/min at 25℃.The results provide an effective way for isolation of lignans.

关 键 词：珠子草 木脂素 分离 高速逆流色谱 

分 类 号：R284.2[医药卫生—中药学]