利用RT-PCR技术扩增绵羊输卵管上皮细胞Ghrelin基因  

Amplification of Ghrelin Gene in Oviduct Epithelial Cells of Sheep by RT-PCR

作　　者：高甜[1,2] 温世勇[1] 钱英红[3] 丛珊[1] 李琦[1] 宋锦[1] 曹贵方[1] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]内蒙古医科大学,内蒙古呼和浩特010110 [3]内蒙古农牧业科学院,内蒙古呼和浩特010031

出　　处：《畜牧与饲料科学》2014年第10期7-9,共3页Animal Husbandry and Feed Science

摘　　要：提取绵羊输卵管上皮细胞总RNA,采用Primer 5.0软件设计引物,运用RT-PCR技术扩增Ghrelin基因。结果显示,经测序和序列比对鉴定,该方法成功地扩增到了大小为200 bp的目的基因,表明RT-PCR技术是检测绵羊输卵管上皮细胞中Ghrelin基因的有效手段,可为今后研究不同激素水平下绵羊输卵管组织中Ghrelin基因的表达量变化及进一步体外试验奠定基础。Total RNA was extracted from oviduct epithelial cells of sheep,primers were designed by Primer 5.0 software,and Ghrelin gene was amplified by RT-PCR. The results showed that 200 bp of aim gene was obtained successfully by RT-PCR, which indicated that RT-PCR technique was an efficient mean to detect expression of Ghrelin gene in oviduct epithelial cells of sheep. The results lay the basis for studying expression change of Ghrelin gene in oviduct tissues of sheep under different hormone levels and further in vitro test.

关 键 词：输卵管上皮细胞 GHRELIN基因 

分 类 号：Q786[生物学—分子生物学] S826.2[农业科学—畜牧学]