时间分辨荧光免疫技术在检测HBV血清学标志物中的应用  

Application of time-resolved fluoroimmunoassay for the detection of HBV serological markers

作　　者：张立营[1] 陈朴[1] 彭宇生[1] 王鹏[1] 

机构地区：[1]宜宾市第一人民医院检验科,四川宜宾644000

出　　处：《国际检验医学杂志》2014年第21期2939-2940,共2页International Journal of Laboratory Medicine

摘　　要：目的比较时间分辨荧光免疫分析技术(TRFIA)和ELISA法在检测HBV血清学标志物的价值。方法分别采用TRFIA和ELISA法检测359例低浓度乙肝表面抗原(HBsAg)标本的HBV血清学标志物,比较两种方法的检测结果的差异。结果TRFIA法检测HBV血清学标志物的各项结果阳性率均高于ELISA法,HBsAg、乙肝e抗原(HBeAg)和乙肝核心抗体(HBcAb)阳性率结果差异有统计学意义(P<0.05),而乙肝表面抗体(HBsAb)、乙肝e抗体(HBeAb)阳性率差异无统计学意义(P>0.05)。结论与ELISA法相比,TRFIA在检测含有低浓度HBsAg标本时具有更高的灵敏度,具有良好的临床应用价值。Objective To compare the value of time-resolved fluorescence immunoassay（TRFIA）and ELISA for the detection of HBV serological markers.Methods To detect the HBV serological markers in 359 samples with low concentrations of HBsAg by TRFIA and ELISA respectively,and to compare the results of the two methods.Results The levels of HBV serological markers detected by TRFIA were higher than those detected by ELISA.The positive rates of HBsAg,HBeAg and HBcAb detected by TRFIA and ELISA were significantly different（P〈0.05）,while those of HBsAb and HBeAb were not（P〉0.05）.Conclusion Compared with ELISA,TRFIA has higher sensitivity in the detection of low HBsAg concentration samples,and it is valuable to be applicated in clinical.

关 键 词：时间分辨荧光免疫分析技术 酶联免疫吸附试验 乙肝表面抗原 血清学标志物 

分 类 号：R446.6[医药卫生—诊断学]