切口周围组织中白细胞介素-1β参与尼古丁戒断大鼠术后切口痛痛觉过敏的研究  

Interleukin-1β in the surrounding incision tissues is involved in postoperative hyperalgesia in rats with nicotine withdrawal

作　　者：逯素芬[1] 于爱兰[1] 刘献文[1] 张宗旺[1] 

机构地区：[1]江苏省麻醉与镇痛应用技术重点实验室,徐州医学院江苏省麻醉学重点实验室,221004

出　　处：《国际麻醉学与复苏杂志》2014年第11期1003-1006,1011,共5页International Journal of Anesthesiology and Resuscitation

摘　　要：目的 探讨切口周围组织中白细胞介素（interleukin,IL）-1β在尼古丁戒断大鼠术后切口痛痛觉过敏中的作用.方法 SD大鼠96只,按随机数字表法分为4组：正常对照组（Naive组,6只）、尼古丁戒断组（NT组,30只）、切口痛组（IP组,30只）和尼古丁戒断切口痛组（NT＋IP组,30只）.NT组、IP组和NT＋IP组大鼠按术前2h和术后2、24、48、72 h分为5个亚组,每个亚组6只.观察各组大鼠上述时间点机械刺激缩足阈值（paw mechanical withdrawal threshold,MWT）、热刺激缩足潜伏期（paw thermal withdrawal latency,TWL）和后爪水肿的变化;取大鼠术侧后爪切口周围皮肤组织,应用酶联免疫分析法检测IL-1β含量.结果 与IP组比较,NT＋IP组大鼠术后2、24、48、72 h MwT和TWL均降低（P＜0.05）,且术侧后爪水肿明显,其中以术后24 h水肿最为显著（P＜0.01）.与IP组各时间点比较,NT＋IP组大鼠相应时间点切口周围组织中IL-1β水平明显增高（P＜0.01）.与术前2 h（19.7±1.9） ng/L比较,NT＋IP组大鼠切口周围组织中IL-1β水平术后各时间点均明显升高（P＜0.01）;术后2h（62.2±3.7） ng/L开始升高,至术后48 h（129.5±6.3） ng/L达高峰,术后72 h开始回降.结论 尼古丁戒断引起大鼠术后痛觉敏感性增高,局部水肿明显;IL-1β参与了尼古丁戒断大鼠术后切口痛痛觉过敏.Objective To explore the contribution of interleukin （IL）-1β in surrounding incision tissues to postoperative hyperalgesia in rats with nicotine withdrawal.Methods Ninety-six SD rats were randomly divided into Naive group （n=6）,NT group （n=30）,IP group （n=30） and NT＋IP group （n=30）.The rats in NT group,IP group and NT＋IP group were divided into 5 subgroups by preoperative 2 h,postoperative 2,24,48 h and 72 h,six in each subgroup.Both paw mechanical withdrawal threshold （MWT） and paw thermal withdrawal latency （TWL） were determined at above time points for every rats.The hind paw edema was also observed.The level of IL-1β in the surrounding incisional tissues was measured by Elisa method.Results Compared with IP group,foot plantar MWT and TWL in NT＋IP group were significantly reduced at postoperative 2,24,48 h and 72 h （P〈0.05）,with most significant decrease at postoperative 48 h （P〈0.01）.Compared with IP group for each time point,IL-1β level in NT＋IP group was significantly increased （P〈0.01）.Compared with preoperative 2 h （19.7± 1.9） ng/L,IL-1β level at all the time points in NT＋IP group were significantly increased （P〈0.01）.The IL-1β level began to rise at postoperative 2 h （62.2±3.7） ng/L,peaked at postoperative 48 h （129.5±6.3） ng/L,and then decreased at postoperative 72 h.Conclusions Nicotine withdrawal can cause postoperative incisional hyperalgesia and hind paw edema in rats.IL-1β is involved in postoperative incisional hyperalgesia in rats with nicotine abstinence.

关 键 词：尼古丁 依赖 戒断 痛觉过敏 白细胞介素-1Β 

分 类 号：R614[医药卫生—麻醉学]