参麦注射液保护D-NNA所致大鼠肾脏氧化损伤的研究  

Protective Effect of Shenmai Injection on N(G)-nitro-D-arginine Induced Oxidative Kidney Damage in Rat

作　　者：张升[1] 陈敏华[1] 徐潘生[1] 顾利强[1] 陈云祥[1] 由振强[1] 辛艳飞[1] 宣尧仙[1] 

机构地区：[1]浙江省医学科学院安全性评价研究中心,浙江杭州310013

出　　处：《中华中医药学刊》2014年第11期2645-2647,I0005,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家"十二五"科技重大专项(2011ZX09301-003);浙江省科技计划项目(2011C37079;2013F20005);浙江省中医药管理局项目(2013ZZ003)

摘　　要：目的:通过研究参麦注射液保护D-NNA所致大鼠肾脏损伤,揭示参麦注射液保护肾脏组织的机制。方法:建立D-NNA诱导大鼠致肾脏损伤模型,用参麦注射液治疗模型组大鼠,通过检测血清生化指标Crea和Bun;肾脏氧化指标MDA、GSH-Px和SOD;一氧化氮水平并结合病理组织学研究,分析D-NNA造模情况和参麦注射液治疗效果。结果:经D-NNA诱导的大鼠血清生化指标Crea和Bun水平均升高,肾脏组织中MDA浓度和GSH-Px活性明显升高,SOD活性明显下降;肾脏组织中NO水平降低,病理学检查证明有严重的肾脏病变。经参麦注射液治疗后所有指标均有改善,病理学检查显示肾脏组织有明显恢复。结论:参麦注射液具有保护D-NNA所致大鼠的肾脏损伤,各氧化指标、NO水平等指标证实其主要机制为参麦注射液在肾脏内具有抗氧化作用。Objective：To investigate the protection of Shenmai Injection for kidney damage induced by D - NNA and to reveal the mechanism of protecting kidney tissue by Shenmai Injection. Method：We constructed a model of kidney damageby intraperitoneally injecting D -NNA, and then Shenmai Injection was used. After that ,we collected serum to detect the Crea and Bun levels. Kidney tissues were gathered for getting the MDA concentration and SOD, GSH - Px activities, NO level expression in kidney tissue would be examined, respectively. The entire index above combined with histopathology was used to evaluate the model of kidney damage induced by D - NNA and the cured effect of Shenmai Injection was as- sessed. Result： The rats, induced by D - NNA, showed significant rise of Bun, Crea and MDA levels and significant decrease of SOD, GSH - Px activities and NO level as compared with control group. In addition, the kidney of rats induced by D -NNA showed remarkable histopathology. After Shenmai Injection treatment, the entire index above trended to become normal, and pathological examination showed the kidney tissue partly recovered. Conclusion：Shenmai Injection can protect kidney damage induced by D - NNA, and the mechanism of protection is antioxidation. Meanwhile, all of the inde- xes studied for oxidation cooperates with histopathological exam tend to ensure this improvement.

关 键 词：参麦注射液 肾脏损伤 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学]