平喘Ⅰ号对呼吸道合胞病毒哮喘鼠气道重塑PDGF-B与ERK信号通路的影响  

Study of Pingchuan I Formula on PDGF-B and ERK Signaling Pathway for Airway Remodeling Asthmatic Mice Infected by Respiratory Syncytial Virus

作　　者：杨季国[1] 马慧娟[1] 徐缨[1] 沈聪玲[1] 李鹏飞[2] 徐王燕 申琳雅 

机构地区：[1]浙江中医药大学附属第三医院,浙江杭州310053 [2]杭州市萧山区中医院,浙江杭州311201

出　　处：《中华中医药学刊》2014年第11期2696-2699,I0009,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金项目(LY12H27011)

摘　　要：目的:研究平喘Ⅰ号在支气管哮喘小鼠气道炎症和气道重塑中的作用。方法:BALB/C雄性小鼠180只,随机分为正常对照组、模型对照组、地塞米松组、平喘Ⅰ号低、中、高剂量组6组,每组30只,每组包括2周、4周、6周组。通过呼吸道合胞病毒(RSV)致敏和激发复制哮喘模型。除正常对照组及模型对照组用等量生理盐水外,其余各治疗组用相应药物给予干预。观察小鼠激发时的症状。通过图像分析软件测定支气管壁厚度(Wat)和平滑肌厚度(Wam),采用实时定量-聚合酶链反应(Real time PCR)测定小鼠肺组织中血小板衍生生长因子(PDGF-B mRNA)的含量,采用免疫印迹法(Western blotting)测定肺组织中细胞外信号调节激酶(extracellular regulated protein kinases,ERK1)的表达水平,直线相关分析法显示PDGF-BmRNA与ERK1的相关性。结果:与正常对照组相比较,各哮喘模型组中Wat和Wam,PDGF-BmRNA含量和ERK1水平均明显增高(P均<0.01)。与模型对照组比较,平喘I号各剂量组和地塞米松组中Wat和Wam,PDGF-BmRNA含量和ERK1水平均明显降低(P<0.01或P<0.05),其中地塞米松各组水平均高于平喘I号低剂量和中剂量组(P<0.05或P<0.01),与高剂量各组相比,地塞米松2周组水平较低(P<0.05),而4周和6周组则增高(P<0.05或P<0.01)。结论:PDGF-B与支气管哮喘气道重塑的程度相关,并与ERK信号传导途径在支气管哮喘气道重塑中起重要作用。平喘Ⅰ号可以降低PDGF-BmRNA的含量和ERK1水平,延缓哮喘小鼠的气道重塑,缓解其症状。Objective ： To investigate the mechanism of Pingchuan I Formula on allergic airway inflammation and airway remodeling in asthmatic mice. Methods：Sixty healthy male BALB/C mice were randomly divided into six groups, which were normal control group, model control group, dexamethasone group, Pingchuan I Formula low, middle and high - dose groups, 10 rats in each. The mice were sensitized and challenged with RSV to establish an asthma model. In addition that the normal control group and model control group received normal saline, all the other groups used the relevant drug intervention. We can observe the activity condition of the mice when challenged. Total bronchial wall thickness （Wat） and smooth muscle thickness （Wam） were measured by image analysis system. RT -PCR and Western Blot were used to measure the level of PDGF - BmRNA and ERK1 in the mice＇ s lung tissues. Analysis on technique about linear correlation was used to measure the correlation between the PDGF - BmRNA and ERK1. Results ： Compared with normal control, asthma model group＇ s Wat and Wam and the level of PDGF - BmRNA and ERK1 in asthma groups were all significantly higher than those in normal control group （P 〈 0.01, respectively） . Compared with the model control group, Pingchuan I Formula each dose groups and dexamethasone group can reduce Wat and Wam and the level of PDGF - BmRNA and ERK1 （P 〈 0.01 or P 〈 0.05 ）. The levels of all dexamethasone groups were significantly higher than that of low and middle - dose groups of Pingchuan I Formula（P 〈 0.05 or P 〈 0.01 ）. Compared with high - dose group, the level of two weeks group of dexamethasone group was lower（ P 〈 0.05 ） but that of the four and six weeks groups were higher（ P 〈 0.05 or P 〈 0.01 ）. Conclusion：Expression of PDGF - B was related with the degree of airway remodeling of asthma. PDGF - B and ERK signal transduction pathway plays an important role in asthma airway remodeling. Pingchuan I Formula can reduce the level of PDGF - BmRN

关 键 词：支气管哮喘 气道重塑 平喘Ⅰ号 细胞外信号调节激酶 血小板衍生生长因子 

分 类 号：R285.5[医药卫生—中药学]