蓝萼香茶菜对脂多糖诱导大鼠发热模型的作用  

Effect of Rabdosia japonica var. glaucocalyx( Maxim. ) Hara on lipopolysaccharide-induced fever in rats

作　　者：褚纯隽[1] 任慧玲[1] 李显伦 严彪[1] 徐乃玉[1] 张健[1] 

机构地区：[1]苏州大学药学院,硕士研究生215123

出　　处：《环球中医药》2014年第11期846-850,共5页Global Traditional Chinese Medicine

基　　金：江苏省高校自然科学研究计划(10KJB350004);2012年苏州市应用基础研究计划(SYS201206);第47批中国博士后科学基金(20100471390)

摘　　要：目的研究蓝萼香茶菜醇提物及其树脂洗脱部位对脂多糖诱导的大鼠发热模型的解热作用及相关炎症因子的影响。方法采用腹腔注射脂多糖(100μg/kg)法建立大鼠发热模型,设空白组、模型组、阳性组、醇提物组和树脂洗脱组。记录灌胃给药后0.5、1、1.5、2、2.5、3、3.5、4、4.5、5、5.5、6、7、8小时大鼠的体温,绘制体温曲线,测定8小时肝组织髓过氧化酶活性和血清中肿瘤坏死因子-α和白介素-6水平。结果模型组大鼠体温呈明显三相热变化趋势,升温峰值(1.97°C)出现在造模后4小时,8小时后体温恢复正常;蓝萼香茶菜醇提物及其树脂洗脱部位显著地降低了大鼠体温峰值;同时有效地抑制了肝组织中髓过氧化酶活性,降低了血清中肿瘤坏死因子-α、白介素-6含量水平;蓝萼香茶菜的树脂洗脱部位在降低大鼠体温峰值方面略胜一筹。结论蓝萼香茶菜醇提物及其树脂洗脱部位对脂多糖诱导的大鼠发热模型有良好的解热作用,可能与抑制髓过氧化酶活性,降低肿瘤坏死因子-α和白介素-6水平有关。Objective To observe the effect of the ethanol extraction （ RJ） and resin eluting frac-tion of RJ （ RJFs） from Rabdosia japonica var. glaucocalyx （ Maxim. ） Hara on lipopolysaccharide （ LPS）-induced febrile and inflammatory factors’ responses in rats. Methods The febrile model of rat was estab-lished by intraperitioneally injecting LPS. Male SD rats were randomly assigned to 5 groups：control group, LPS （100 μg/kg） group, Aspirin, RJ （305 mg/kg） and RJFs （305 mg/kg） groups. After LPS challenge the temperature is recorded every 30 min, it lasted for 6 h, then 7 h and 8 h. Then, myeloperoxidase （ MPO） activities in liver tissues was examined, the levels of inflammatory cytokine tumor necrosis factor-α（TNF-α） and interleukin-6 （IL-6） in serum are measured using ELISA. Results The rats of model group showed obvious three-phase fever and the heating peak value （1. 97 ℃） appeared at 4 h. It is found that both RJ and RJFs significantly decreased the body temperature of rats, and at 8 h following LPS challenge effectively inhibited MPO activity in liver tissues, TNF-α and IL-6 concentration levels in serum. Com-pared with RJ, RJFs was slightly better at decreasing the body temperature. Conclusion RJ and RJFs have the action of antipyretic effect, and it may be related to inhibiting the level of MPO, TNF-αand IL-6.

关 键 词：蓝萼香茶菜 脂多糖 解热 炎症因子 

分 类 号：R285[医药卫生—中药学]