云南省西部边境新分离蚊媒病毒分子生物学特征分析  

Molecular characterization of mosquito-borne viruses strains newly isolated in Western Yunnan Province along the border of China and Myanmar, China

作　　者：冯云[1] 付士红[2] 杨卫红[4] 章域震[1] 杨捷[3] 刘永华[1] 董朝良[3] 李轼[4] 高晓艳[2] 江伟[3] 尹小雄 杨世朝[3] 郭天巧[3] 张保森[3] 尹正留 梁国栋[2] 张海林[1] 

机构地区：[1]大理云南省地方病防治所/云南省病毒立克次体研究中心,云南671000 [2]中国疾病预防控制中心病毒病预防控制所 [3]德宏州疾病预防控制中心 [4]瑞丽市疾病预防控制中心

出　　处：《国际病毒学杂志》2014年第5期193-198,共6页International Journal of Virology

基　　金：中美新发和再发传染病合作项目（No.U19-GH000004）

摘　　要：目的分析云南中缅边境地区新分离蚊媒病毒的分子生物学特征，以分析其遗传进化特征。方法2010年1—12月在云南省德宏州芒市和瑞丽市监测点每月一次用诱蚊器采集蚊虫，用BHK-21和C6／36细胞分离病毒，阳性分离物用RT—PCR法进行鉴定及序列测定和分析。结果从采获的7属42种69209只蚊虫中分离到13株流行性乙型脑炎病毒（Japaneseencephalitisvirus，JEV），4株盖塔病毒（Getahvirus，GETV），1株西藏环状病毒（Tibetorbivims，TIBOV），7株淡色库蚊浓核病毒（Culexpipienspallensdensovirus，CppDNV）。JEVE基因进化分析证实，新分离的13株JEV均属基因I型，与云南其它地区和邻近省份流行株亲缘关系较近，且抗原和毒力位点未发生明显改变。4株GETVE2基因与云南其它地区分离株同源性高，亲缘关系近。新分离环状病毒的VPl基因与TIBOV亲缘关系最近，而与云南环状病毒（Yunnanorbivirus）存在明显差异。7株CppDNV的NSl基因与以往云南及其他省区流行株具有较高的同源性和亲缘关系。结论首次证实该地区存在基因I型JEV、GETV、TIBOV和CppDNV流行，这些流行株均具有较为稳定的遗传特征和地域特征。Objective To analysis the molecular characterization of mosquito-borne viruses strains newly isolated in Wastern Yunnan Province along the border of China and Myanmar, China. Methods Mosquito samples was carried out with mosquito traps from Mangshi and Ruili counties of Dehong prefecture, Yunnan Province, once every mouth from Jan to Dec, 2010. Mosquitoes were BHK-21 and C6/36 cells cul- ture for viral isolation, and positive isolates were identified using RT-PCR and sequence analysis. Results A total of 69,209 mosquitoes comprised of 42 species representing seven genera were collected. Thirteen strains of Japanese encephalitis virus （JEV）, four strains of Getah virus （ GETV）, one strain of Tibet orbivir- us （TIBOV）, seven strains of Culex pipiens pallens densovirus （CppDNV） were isolated from the mosquito pools. Phylogenetie analysis of E gene showed that all the 13 JEV strains belonged to genotype I , and were closely related to isolates from other area of Yunnan Province and neighboring Chinese provinces, the mostcritical sites associated with viral virulence and antigenicity were conserved. The E2 gene of the four GETV strains shared high homology and closely relative with sequences of other GETVs from Yunnan Province. The VP1 gene of the newly-isolated Orbivirus shared closely related with sequences of TIBOV from Tibet Prov- ince, China, however, distinct difference with sequences of Yunnan orbivirus isolated from Yunnan Prov- ince. The NS1 gene of the seven CppDNV strains shared high homology and closely relative with sequences of other CppDNVs from Yunnan Province and other provinces of China. Conclusion Genotype I JEV, GETV, TIBOV and CppDNV were the first report in this area, suggested that these virus strains harbored relatively stable genetic characteristics and distinct regional features.

关 键 词：流行性乙型脑炎病毒 盖塔病毒 西藏环状病毒 淡色库蚊浓核病毒 

分 类 号：R181.3[医药卫生—流行病学]