厌氧消化器中甲烷古菌的分离与纯化  

Separation and Purification of Methanoarchaea in the Anaerobic Digester

作　　者：王彬 邓功成[2] 苏小二 

机构地区：[1]贵州省遵义县枫香中学,贵州遵义56112 [2]黔南民族师范学院生命科学系,贵州都匀55800

出　　处：《黔南民族师范学院学报》2014年第5期112-116,共5页Journal of Qiannan Normal University for Nationalities

基　　金：黔教高发[2012]426号黔南民族师范学院微生物学重点学科[2011.6];黔南民族师范学院教学质量工程项目院教发[2011]5号

摘　　要：目的:探索分离纯化严格厌氧微生物的方法。方法:运用微生物学基础理论和Hungate厌氧操作系统,采用滚管分离培养方法对厌氧消化器中甲烷菌进行富集、滚管分离纯化。结果:获得甲烷菌纯培养。结论:Hungate滚管分离培养技术方法可靠,分离的效果好,投资不大,适用于各种厌氧微生物的分离和培养。The objective is to explore the way of separating and purifying anaerobic microorganism. This paper uses the basic theories of microbiology and the operating system of Hungate anaerobe and a- dopts roiling tube separation to enrich, roll -tube, separate and purify the methanoarchaea in the an- aerobic digester. The results show that the purely cultivated methanoarchaea is obtained. The conclu- sion demonstrates that roll tube cuhivation method is reliable, the effect of separation is good, the in- vestment is small, therefore, this technology is suitable for separation and cultivation of all kinds of an- aerobic microorganisms.

关 键 词：甲烷古菌 Hungate除氧系统 富集 滚管分离 

分 类 号：Q939[生物学—微生物学]