白花蛇舌草总多糖的分离纯化、结构鉴定及初步免疫活性分析  

Effect of Preliminary Immune Activity and Structural Identification of a Polysaccharide Extracted from Oldenlandia diffusa

作　　者：马河[1,2] 程艳琳 张金杰 曹广尚[3] 杨培民[3] 

机构地区：[1]山东中医药大学,济南250355 [2]山东省药学科学院化学药物重点实验室,济南250101 [3]山东中医药大学附属医院,济南250011

出　　处：《中国实验方剂学杂志》2014年第22期37-40,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81274052);山东省自然科学基金项目(ZR2011HL043);山东省优秀中青年科学家科研奖励基金项目(BS2013YY052)

摘　　要：目的:分离纯化白花蛇舌草总多糖,研究其对环磷酰胺致免疫低下小鼠的免疫增强活性。方法:采用超滤技术分离纯化白花蛇舌草总多糖,高效凝胶渗透色谱-示差检测器方法测定相对分子质量,柱前衍生化HPLC分析其单糖组成;采用环磷酰胺致免疫低下小鼠碳粒廓清试验研究其免疫活性。结果:从白花蛇舌草中分离得到1个单一多糖组分ODP-1,其相对分子质量20.88 k Da,单糖组成为甘露糖-鼠李糖-半乳糖醛酸-葡萄糖-半乳糖-阿拉伯糖,摩尔比0.005∶0.033∶0.575∶1.000∶0.144∶0.143;免疫活性试验结果表明,ODP-1可升高环磷酰胺致免疫低下小鼠廓清指数、吞噬指数、胸腺指数和脾指数。结论:白花蛇舌草多糖ODP-1为均一多糖,具有免疫增强活性。Objective： The polysaccharide was separated and purified from Oldenlandia diffusa and the immuno-enhancing activity was evaluated in immunosuppression mice by intraperitoneal administration. Method：The crude polysaccharides were separated and purified by tangential-flow ultrafiltration,then the relative molecular weight was measured by gel column chromatography. The structure was analyzed by pre-column derivatization HPLC. The activity of the homogeneous polysaccharide was investigated by carbon clearance on immunosuppression mice. Result： A new homogeneous polysaccharide named ODP-1 was obtained from O. diffusa. The relative molecular weight of ODP-1 was about 20. 88 k Da. ODP-1 was composed of mannose,rhamnose,galacturonic acid,glucose,galactose and arabinose with the molar ratio of 0. 005 ∶ 0. 033 ∶ 0. 575 ∶ 1. 000 ∶ 0. 144 ∶ 0. 143. Immune activity indicated that ODP-1 can improve the clearance index,phagocytic index,the index of thymus and spleen of immunosuppression mice. Conclusion： ODP-1 from O. diffusa is a homogeneous polysaccharide with immunoenhancing activity.

关 键 词：白花蛇舌草 多糖 切向流超滤 相对分子质量 碳粒廓清 

分 类 号：R284.1[医药卫生—中药学]