苦豆子种子生物碱类化学成分及细胞毒活性  

Alkaloids Constituents of Sophora alopecuroides Seed and Their Cytotoxic Activity

作　　者：姚雯[1] 孙培环[1] 徐晓敏[1] 李雅娟[1] 袁呈山[2] 王振华[1,3] 

机构地区：[1]石河子大学药学院/新疆特种植物药资源教育部重点实验室,新疆石河子832002 [2]兰州大学功能有机分子化学国家重点实验室,兰州730000 [3]烟台大学生命科学学院,山东烟台264005

出　　处：《中国实验方剂学杂志》2014年第22期95-99,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(11175222);教育部新世纪优秀人才支持计划(NCET-10-0967)

摘　　要：目的：研究苦豆子种子的化学成分并对其抗肿瘤活性进行测试。方法：采用常规的硅胶柱层析（CC）,葡聚糖（Sephadex LH-20）柱层析,和重结晶等实验手段对其进行了分离纯化,利用波谱技术（1H-NMR,13C-NMR）及与文献数据对照,对分离的化合物进行了结构鉴定;并采用MTT测定部分生物碱单体化合物体外对C6肿瘤细胞的细胞毒活性。结果：共分离出7个生物碱类化合物,分别鉴定为：苦参碱（1）,氧化苦参碱（2）,槐果碱（3）,9α-羟基槐果碱（4）,氧化槐果碱（5）,9α-羟基槐胺碱（6）,（-）-13,14-去氢槐定碱（7）,1个非生物碱化合物3-吲哚甲醛（8）。结论：化合物8为首次从该属植物中分离得到。细胞毒活性测试结果表明,化合物1-3和5对鼠脑胶质瘤C6细胞显示了较强的生长抑制活性。Objective： To investigate the chemical constituents in seed of Sophora alopecuroides and to determine their antitumor activities in vitro. Method： Compounds were isolated and purified by means of silica gel and Sephadex LH-20 gel columm chromatography,and recrystallization etc. Their structures were elucidated using spectroscopic analysis including1H-NMR,13C-NMR and spectral data. The cytotoxic activities of the alkaloids compounds on C6 cell in vitro were evaluated by MTT method. Result： Seven compounds were isolated from the ethanol extract of the seed of S. alopecuroides. Their structrues were identified as（ ＋）-matrine（ 1）,（ ＋）-oxymatrine（ 2）,（-）-sophocarpine（ 3）,（-）-9α-hydroxysophocarpine（ 4）,（ ＋）-oxysophocarpine（ 5）,（-）-9α-hydroxysophoramine（ 6）,（-）-13,14-dehydrosophoridine（ 7）,and a non-alkaloid named indolyl-3-carbaldehyde（ 8）. Conclusion： The compond 8 was isolated from the genus sophora for the first time.Compounds 1-3 and 5 showed significant inhibitory activity towards C6 glioma cells.

关 键 词：苦豆子 化学成分 生物碱 结构鉴定 细胞毒活性 

分 类 号：R284.2[医药卫生—中药学] R284.1[医药卫生—中医学]