地骨皮水提液对脂多糖诱导小鼠急性肺损伤的保护作用机制  

Lycium chinense Water Extract Attenuates Acute Lung Injury Induced by Lipopolysaccharide in Mice

作　　者：张天柱[1] 张景龙[1] 郝彬彬[1] 徐江[1] 

机构地区：[1]长春中医药大学,长春130117

出　　处：《中国实验方剂学杂志》2014年第22期147-150,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81273883);吉林省中医药科技项目(2012-083)

摘　　要：目的:探讨地骨皮水提物对内毒素(lipopolysaccharide,LPS)诱导小鼠的急性肺损伤(acute lung injury,ALI)保护作用。方法:小鼠LPS(5 mg·kg-1ip)复制ALI动物模型。将小鼠随机分为空白组、模型组、地塞米松组(2 mg·kg-1)、地骨皮水提液高、低剂量组(20,10 g·kg-1),腹腔注射给药。观察各组肺组织病理学改变,测量肺湿质量/干质量,支气管肺泡灌洗液(BALF)中性粒细胞百分比、肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、炎症因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量及肺组织中SOD与MDA蛋白表达。结果:地骨皮水提液能降低肺泡灌洗液(BALF)中炎症细胞的数目,能显著降低肺湿质量/干质量,与空白组相比,模型组肺组织MDA含量升高(P<0.01),SOD活性降低(P<0.05),MDA蛋白水平显著升高,SOD蛋白水平显著下降(P<0.01);与模型组比较,地骨皮水提液组BALF中SOD活性显著升高(P<0.05),MDA蛋白水平降低(P<0.05),并可有效减轻LPS所致肺组织病理学变化。结论:地骨皮水提液可减轻LPS所致急性肺组织损伤。Objective： To determine the protective effect of Lycium chinense water extract（ LCM） on acute lung injury（ ALI） induced by lipopolysaccharide（ LPS）. Method： The BABL / c mice were randomly allocated into control group,LPS model group,dexamethasone（ 2 mg·kg^-1） group,LCM（ 10 g·kg^-1）,and LCM（ 20 g·kg^-1） group by intraperitoneal injection. The bronchoalveolar lavage fluid（ BALF） samples were analyzed for the concentration of numbers of neutrophilic, superoxide dismutase（ SOD）, malondi aldehyde（ MDA）,interleukin-6（ IL-6） and tumor necrosis factor alpha（ TNF-α）,and the lung samples were taken for histologic evaluation and for determination of wet-to-dry（ W / D） lung weight,the protein levels of SOD and MDA were detected by Western blot analysis. Result： Pretreatment with LCM prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight / dry weight ratio,in total leukocyte number and neutrophil percent in the BALF,and in MDA activity of lung in dose-dependent manners,and in IL-6,the TNF-α in the BALF and MDA protein in lung and increased SOD protein in lung（ P〈0.01）,compared with model the group.At the same time,compared with the blank group,pretreatment with LCM also significantly improved the SOD activity（ P〈0.05） and decreased the MDA activity in lung（ P〈0.05）. Conclusion： These results showed that anti-inflammatory effects of LCM against the LPS-induced ALI.

关 键 词：地骨皮水提液 炎症 内毒素诱导急性肺损伤 超氧化物歧化酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]