两种杜仲黄酮类化合物对成骨细胞OPG/RANKL及成骨相关转录因子的影响  

Effects of Two Kinds of Eucommia ulmoides Flavonoids on Osteoblast OPG / RANKL and Osteogenic Transcription Factor

作　　者：兰波[1] 刘亭[1] 谢玉敏[1] 王爱民[2] 李勇军[1] 

机构地区：[1]贵阳医学院贵州省药物制剂重点实验室,贵阳550004 [2]民族药与中药开发应用教育部工程研究中心,贵阳550004

出　　处：《中国实验方剂学杂志》2014年第22期180-184,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2013BAI11B01);贵州省科技重大专项(黔科合重大专项字[2012]6009号);贵州省中药现代化专项项目(黔科合中药字[2013]5062号)

摘　　要：目的:研究杜仲黄酮类化合物紫云英苷和黄芩素对成骨细胞特异性转录因子Osterix及破骨细胞抑制因子与核因子κB受体活化因子配体比值(OPG/RANKL)的影响。方法:采用MC3T3-E1 Subclone 14成骨细胞,不同浓度紫云英苷和黄芩素和维生素D3组加入质量浓度为30 mg·L-1作用细胞24 h后,用MTT法检测细胞增殖情况,成骨细胞接种于24孔板后,空白组加入培养基,给药组分别加入质量浓度为80 mg·L-1(高浓度),40 mg·L-1(中浓度),7.5 mg·L-1(低浓度)的含紫云英苷或黄芩素,作用24 h后,ELISA法检测钙离子活性,蛋白免疫印迹法检测Osterix,OPG以及RANKL的蛋白表达水平。结果:与空白组相比,紫云英苷和黄芩素可后明显促进MC3T3-E1 Subclone 14成骨细胞的增殖(P<0.05);明显上调OPG和Osterix的表达(P<0.05),同时能降低RANKL的表达(P<0.05)。结论:杜仲黄酮类化合物紫云英苷和黄芩素能促进MC3T3-E1Subclone 14成骨细胞增殖,并上调Osterix和OPG/RANKL。Objective： To investigate the effects of Eucommia ulmoides flavonoids astragalin and baicalein on the expression of osteoblast specific transcription factor Osterix and the ratio of osteoproteger and receptor activator of nuclear factor kappa B ligand（ OPG / RANKL）. Method： MC3T3-E1 Subclone 14 osteoblasts were cultured with different concentrations of astragalin,baicalein and vitamin D3 for 24 h. MTT detection method was used to detect cell proliferati. The expression of Ca2 ＋was detected by ELISA. The expressions of OPG,RANKL,OST were detected by Western blotting. Result： Compared with the control group,astragalin and baicalein can significantly promote the proliferation of MC3T3-E1 Subclone 14（ P〈0.05）,increase the expression of OPG and Osterix obviously（ P〈0.05）,and reduce the expression of RANKL（ P〈0.05）. Conclusion： It shows that astragalin and baicalein from E. ulmoides flavonoids can promote MC3T3 E1 Subclone 14 osteoblast proliferation,and raised Osterix and OPG / RANKL.

关 键 词：杜仲 骨保护素 成骨细胞 成骨相关转录因子 蛋白免疫印迹法 

分 类 号：R285.5[医药卫生—中药学]