模拟酸雨胁迫对菲白竹生理特性的影响  

Effects of Simulated Acid Rain on Physiological Characteristics of Pleioblastus fortunei

作　　者：王涛[1] 张珊珊[1] 谢寅峰[1] 田雪瑶[1] 唐颂豪 季晓钒 

机构地区：[1]南京林业大学,南京210037

出　　处：《东北林业大学学报》2014年第11期17-21,共5页Journal of Northeast Forestry University

基　　金：江苏高校优势学科建设工程资助项目(PAPD);江苏省高校自然科学基金项目(05KJB220053)

摘　　要：以园林观赏竹种菲白竹为材料,采用人工模拟酸雨的方法研究不同强度酸雨(p H=4.0、p H=3.0、p H=2.0)胁迫对菲白竹生理特性的影响。结果表明:不同强度的酸雨胁迫均导致菲白竹叶片出现不同程度的伤害症状,受伤害程度随着酸雨p H值的降低而加剧。胁迫解除初期,叶片可溶性蛋白质量分数和叶绿素相对含量(SPAD值)均低于对照,而丙二醛(MDA)质量摩尔浓度,质膜透性以及超氧化物歧化酶(SOD)、过氧化物酶(POD)活性较对照出现不同程度的升高,变化幅度随胁迫强度的增加而增加;不同生理指标对胁迫的敏感程度有所不同,可溶性蛋白质量分数、叶绿素相对含量、质膜透性等可作为反映酸雨胁迫下菲白竹受伤害程度的重要依据。经过1个月左右的恢复期,p H=4.0胁迫处理组的大多数指标能恢复至对照水平;p H=3.0处理组有一定的恢复趋势,但恢复程度不明显;而p H=2.0胁迫组的各项指标均不能恢复,且与对照差异显著(P<0.05)。说明菲白竹对模拟酸雨p H=4.0的胁迫处理表现出较强的自我修复能力;对p H=3.0酸雨胁迫具有一定的修复作用;但在p H=2.0处理下,则产生不可逆伤害,无法通过自我调节恢复,这与外部形态的观察结果一致。高频度强酸雨(p H=2.0)处理下,菲白竹叶片伤害的主要原因可能是由过量H+累积对植株造成的直接伤害引起的;而低酸度(p H=4.0)处理产生的生理伤害及其修复与活性氧代谢调节有关。Leaves of Pleioblastus fortunei, an ornamental bamboo species, were sprayed with simulated acid rain （ SAR） with different levels of pH （ 4.0, 3.0, 2.0） to study the effects of SAR on leaf physiological characteristics.The SAR with dif-ferent intensity could result in damage symptoms appeared on the leaves.The lower the pH value was, the severer the dam-age was.During the early stage of stress relief, the values of Pr and SPAD were below to control and MDA, MP, SOD and POD were higher than control, whose ranges of the change increased significantly as the stress became deeper.Pr, MDA and MP could play as an important basis reflecting the damage degrees under SAR stress because of the sensitivities to stress.After one month recovery, the most of indexes of pH of 4.0 could return to control.pH of 3.0 could recover but not obviously.All the indexes of pH of 2.0 could not return to control and with significant differences （ P〈0.05） .P.fortunei can adapt to pH of 4.0 SAR stress and stand pH of 3.0 SAR in a certain degree, but suffers irreversible damage during pH of 2.0 SAR, which is agree with morphological observation.Under high frequent pH of 2.0 SAR, the leaf damage of P.for-tunei may be resulted directly from the accumulation of excessive H＋.However, the physiological damage and repair pro-duced by pH of 4.0 SAR is related to the regulation of reactive oxygen species metabolism.

关 键 词：模拟酸雨 菲白竹 生理响应 抗性 

分 类 号：Q945.78[生物学—植物学]