欧美杨溃疡病菌在侵染寄主过程中的动态变化  

Dynamic Changesof Lonsdalea quercina subsp.populi in Infection Process of Populus×euramericana

作　　者：商靖[1] 李永[2] 李爱宁[1] 贺伟[1] 郭利民 常聚普 

机构地区：[1]省部共建森林培育与保护教育部重点实验室(北京林业大学),北京100083 [2]中国林业科学研究院森林生态环境与保护研究所 [3]河南省濮阳市林业科学院

出　　处：《东北林业大学学报》2014年第11期120-123,137,共5页Journal of Northeast Forestry University

基　　金：林业公益性行业专项(201104054);国家自然基金青年基金项目(31200486)

摘　　要：采用Real－timePCR和病理组织切片相结合的方法，分析了欧美杨溃疡病病原菌（Lonsdaleaquercinasubsp．populi）侵染欧美杨（Populus＆#215;euramericana‘zhonglin46’）后不同时间的种群量及其变化。结果表明：接种3d后植株内己有一定的菌量积累，从结构上看，对植物尚未造成明显的伤害。但从接种第5天开始，菌量明显增加，部分细胞破裂，植株显症。接种9～15d，菌量增加到峰值，并进入稳定期，韧皮部组织细胞破裂明显，薄壁细胞解离，植株显症严重。应用Real－timePCR分子定量法可直接对欧美杨植株体内的病原菌进行动态定量研究。We quantified bacterial population of Lonsdalea quercina in Populus×euramericana‘Zhonglin 46’ and disease symp-tom occurrence in the infection process of the pathogen by using real-time PCR and histopathology.The bacterial accumula-tion in number within Poplar branch bark tissue was detected at 3-day post-inoculation （dpi）, while no disease symptom-soccurred.Then, the bacterial density within Poplar increased significantly with some cells in cortical layer broken, initial symptom occurred 5 dpi.Bacterial numbers reached a peak and maintained a high population 9-16 dpi, when the cell in the phloem was broke seriously, and parenchyma cells were disaggregated.The plants displayed severe disease symptoms. Therefore, real-time PCR assays can be used to quantify the pathogen in infected plant tissues.

关 键 词：欧美杨溃疡病 REAL-TIME PCR 组织病理学 

分 类 号：S763.13[农业科学—森林保护学] S792.11[农业科学—林学]