几种杀虫剂对分月扇舟蛾离体酶活性的影响  

Effects of Several Pesticides on the Activities of in vitro Enzymes in Clostera anastomosis

作　　者：刘晓露[1] 古丽吉米拉.米吉提[1] 王志英[1] 吴红渠 

机构地区：[1]东北林业大学,哈尔滨150040 [2]湖北生物农药工程研究中心

出　　处：《东北林业大学学报》2014年第11期128-133,共6页Journal of Northeast Forestry University

基　　金："十二五"农村领域国家科技计划课题(2011BAD37B01 4);林业公益性行业科研专项(201104069)

摘　　要：以分月扇舟蛾(Clostera anastomosis)3龄幼虫为研究对象,比较了几种杀虫剂对其离体保护酶(SOD和CAT)和解毒酶(Car E和GST)活性的影响。结果表明:0.100 mg·L-1高效氯氟氰菊酯、0.010 mg·L-1吡虫啉、0.001 mg·L-1灭幼脲对CAT活性的抑制作用较强,抑制率分别为63.50%、68.89%和62.11%,而敌敌畏、氧化乐果对CAT活性的抑制作用较小。0.100 mg·L-1高效氯氟氰菊酯对SOD活性的抑制作用最强,抑制率为60.82%,氧化乐果对SOD活性有较强的促进作用,激活率可达168.72%(10.000 mg·L-1)。10.000 mg·L-1敌敌畏对Car E的抑制作用最强,抑制率为52.63%,1.000 mg·L-1高效氯氟氰菊酯对Car E的激活作用最强,激活率为43.06%。供试杀虫剂对GST活性均有抑制作用,1.000 mg·L-1灭幼脲对GST抑制率可达51.75%。由此可见,离体保护酶和解毒酶的活性与杀虫剂的类型有直接关系,并且同一类型不同化学结构的杀虫剂对离体酶活性的影响存在显著差异。We studied the effects of several pesticides on the activities of SOD, CAT, CarE and GST in three instars of Clostera anastomosis.The activities of CAT were highly inhibited by 0.1 mg· L-1 lambda-cyhalothrin, 0.010 mg· L-1 imidacloprid, and 0.001 mg· L-1 diflubenzuron, and the inhabitation rate were 63.50%, 68.89%and 62.11%, respectively.However, the activities of CAT were lowest inhibited by dichlorvos and omethoate, and highest inhibited by 0.1 mg· L-1 lambda-cy-halothrin.The inhabitation rate of 0.100 mg· L-1 lambda-cyhalothrin was 60.82%.The activities of SOD were highly in-creased by 10.000 mg· L-1 omethoate, and the increasing rate was 168.72%.The activities of CarE were highly inhibited by 10.000 mg· L-1 dichlorvos, and the increasing rate was 52.63%.The activities of CarE was highest increased by 1.000 mg· L-1 lambda-cyhalothrin, and the increasing rate of 1.000 mg· L-1 lambda-cyhalothrin was 43.06%.The activities of GST were all inhibited by the tested pesticides and the inhabitation rate of 1.000 mg· L-1 lambda-cyhalothrin reached 51.75%. Thus, the activities of protective and detoxification enzymes have direct relation to the type of pesticides, and the effects on the activities of protective and detoxification enzymes are significant different by the same type of pesticides with different chemical structures.

关 键 词：分月扇舟蛾 过氧化氢酶 超氧化物歧化酶 羧酸酯酶 谷胱甘肽-S-转移酶 

分 类 号：S763.306.7[农业科学—森林保护学] S763.42[农业科学—林学]